Anti-Hepatitis B Virus Core Antigen antibody [H3A4] (ab8255)




Our Abpromise guarantee covers the use of ab8255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.


  • RelevanceHepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • Cellular localizationCapsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • Alternative names
    • C antibody
    • Capsid protein antibody
    • Core and e antigen antibody
    • Core antigen antibody
    • Core protein antibody
    • HBc antibody
    • HBcAg antibody
    • HBVgp4 antibody
    • Hepatitis B Virus core antigen antibody
    • p21.5 antibody
    • precore/core protein antibody
    see all

References for Anti-Hepatitis B Virus Core Antigen antibody [H3A4] (ab8255)

ab8255 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Serum (from HBV patients)
Specification from HBV patients
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Type Sandwich (Capture)

Abcam user community

Verified customer

Submitted Dec 31 2009

I have contacted our accounting department and a refund will be issued for your order for ab8255. Please let me know if you have any further questions.

Thank you very much for re-sending the information. As you were able to detect the correct sized band with the polyclonal HBcAg antibody, but not with ab8255, it's possible that there is a problem with the particular vial that you received. I can send ...

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Thank you for your patience and I'm sorry to hear that you are experiencing difficulty with this antibody. I'm afraid that I'm unable to open the pdf that you attached - can you send it as a jpg? Also, what dilutions of ab8255 did you use? What was you...

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Here is the information that I could obtain regarding ab8639, ab8638, and ab8637. The recommended starting dilutions are 1:1,000 and down in 10-fold increments. As for detection, any known detection method would work. Once again, those mabs had being c...

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I have received the following information regarding ab8255 and ab2045, but will have to get back to you about the other antibodies. The originator has said that they tested the antibodies as pairs in internal ELISA. Possible pairs are H6F5 (ab2045) - H...

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Thank you for your enquiry. I was able to obtain the following information regarding the use of these antibodies in ELISA. Ab8255 and Ab2045: These antibodies have been tested in ELISA with the native antigen. The antibodies can be used as a pair in...

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The antibody was purified from ascites.

Dear Purmima I am afraid that the epitope isn't known for this antibody, however I can tell you that it is the same antigen as the H3B3 clone. I am sorry we can't give you any more information. However, if you do manage to find any more informat...

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