Hepatitis B virus Hepatitis B Surface Antigen full length protein (ab113610)

Overview

  • Product nameHepatitis B virus Hepatitis B Surface Antigen full length protein
  • DescriptionHepatitis B virus Hepatitis B Surface Antigen full length protein

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • AccessionQ9PX80
    • SpeciesHepatitis B virus
    • Molecular weight25 kDa
    • Amino acids1 to 226
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab113610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab113610 reacts with Human HBV positive serum
  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Purity> 90 % by SDS-PAGE.
    ab113610 is purified by Nickel affinity and Ion Exchange chromatography
  • FormLiquid
  • Additional Notesab113610 reacts with Human HBV positive serum
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

    pH: 7.20
    Constituents: 0.1% Phosphate Buffer, 0.44% Sodium chloride, 50% Glycerol

General info

  • Alternative names
    • HBsAg
    • Hep B surface antigen
    • Hepatitis B Virus major surface antigen
    • Hepatitis B virus S antigen
    • Large envelope protein
    • Large surface protein
    • LHB
    • Major surface antigen
    see all
  • RelevanceHepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
  • Cellular localizationCell Membrane

References for Hepatitis B virus Hepatitis B Surface Antigen full length protein (ab113610)

ab113610 has not yet been referenced specifically in any publications.

Product Wall



Per the lab, this product is an aggregated protein that without heating would run as monomers, dimers and multimers on Western Blots and in SDS-PAGE.

Per the lab, the antigen assembles into particles and they recommend sonication, but please be advised, they have not performed this.

Here are a few specific sELISA protocol details I was able to find:

ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL. Read More



If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

We have not yet de...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"