Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)

Overview

  • Product nameAnti-Hepatitis B Virus pre S2 Antigen antibody [S26]
    See all Hepatitis B Virus pre S2 Antigen primary antibodies
  • Description
    Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen
  • SpecificityThis antibody reacts with HBV Large and Middle Surface Antigen but not small forms (both glycosylated and non glycosylated species).
  • Tested applicationsSuitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WB, ICC/IFmore details
  • Immunogen

    Purified serum HBV surface antigen

  • Epitopeaa positions 131-139 (adw nomenclature)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purification notesBioreactor harvest: Antibodies generated as bioreactor harvest produce less background as they are less contaminated with other proteins compared to ascites.
  • ClonalityMonoclonal
  • Clone numberS26
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-FoFr
IHC-Fr
Immunomicroscopy
IP
IHC-P
WB
ICC/IF
  • Application notesFor western blotting use at 1:1000
    For immunohistochemistry use at 1:100
  • Target

    • RelevanceThe human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma. The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced. The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes.
    • Cellular localizationVirion membrane (By similarity).
    • Alternative names
      • HBV pre S2 Antigen antibody
      • HBV pre S2 protein antibody
      • Hepatitis B Virus pre S2 protein antibody
      • PreS2/S antigen antibody
      • S antibody
      see all

    Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] images

    • All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution

      Lane 1 : Whole cell lysate prepared from HEK cells
      Lane 2 : Whole cell lysate prepared from HEK cells transfected with pCI M
      Lane 3 : Whole cell lysate prepared from HEK cells transfected with pCI M

      Lysates/proteins at 50 µg per lane.

      Secondary
      Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 33,36,39 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 minutes

      Image courtesy of Alina Macovei by Abreview.

    • Immunofluorescence analysis of mutant pHBV-env–transfected Huh-7 cells, staining Hepatitis B Virus pre S2 Antigen with ab8635.

      Cells were fixed with 4% paraformaldehyde and permeabilized with with saponin 0.1%. Cells were incubated with primary antibody (1/500)  for 2 hours at room temperature. An Alexa Fluor®555–conjugated goat anti-mouse IgG (1/1000) was used as the secondary antibody.

    References for Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)

    This product has been referenced in:
    • Draz MS  et al. Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus. ACS Nano 6:7634-43 (2012). Read more (PubMed: 22934963) »
    • Villet S  et al. In vitro characterization of viral fitness of therapy-resistant hepatitis B variants. Gastroenterology 136:168-176.e2 (2009). ICC/IF . Read more (PubMed: 18996386) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeK cells)
    Loading amount 50 µg
    Specification HeK cells
    Gel Running Conditions Reduced Denaturing (10)
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Username

    Mrs. Alina Macovei

    Verified customer

    Submitted Apr 15 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (transfected Huh7)
    Specification transfected Huh7
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3
    Username

    Miss. Nadia Warner

    Verified customer

    Submitted Feb 07 2006

    I can confirm that the antibody ab8635 recognizes large and middle but not small forms of HBsAg. Both glycosylated and non glycosylated species are reactive. Please do not hesitate to contact us again if you need further information,

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"