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Purified serum HBV surface antigen
Our Abpromise guarantee covers the use of ab8635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Image courtesy of Alina Macovei by Abreview.
Immunofluorescence analysis of mutant pHBV-env–transfected Huh-7 cells, staining Hepatitis B Virus pre S2 Antigen with ab8635.
Cells were fixed with 4% paraformaldehyde and permeabilized with with saponin 0.1%. Cells were incubated with primary antibody (1/500) for 2 hours at room temperature. An Alexa Fluor®555–conjugated goat anti-mouse IgG (1/1000) was used as the secondary antibody.