The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For western blotting use at 1:1000
For immunohistochemistry use at 1:100
The human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma.
The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced.
The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC).
Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes.
Virion membrane (By similarity).
HBV pre S2 Antigen antibody
HBV pre S2 protein antibody
Hepatitis B Virus pre S2 protein antibody
PreS2/S antigen antibody
Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)Image courtesy of Alina Macovei by Abreview.
All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HEK cells Lanes 2-3 : Whole cell lysate prepared from HEK cells transfected with pCI M
Lysates/proteins at 50 µg per lane.
Secondary All lanes : Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)Image from Villet S et al., Gastroenterology, 136, 168-176.e2. Fig 3.; doi: 10.1053/j.gastro.2008.09.068. Epub 2008 Oct 7. with permission from Elsevier.
Immunofluorescence analysis of mutant pHBV-env–transfected Huh-7 cells, staining Hepatitis B Virus pre S2 Antigen with ab8635.
Cells were fixed with 4% paraformaldehyde and permeabilized with with saponin 0.1%. Cells were incubated with primary antibody (1/500) for 2 hours at room temperature. An Alexa Fluor®555–conjugated goat anti-mouse IgG (1/1000) was used as the secondary antibody.