Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (ab9193)


  • Product nameAnti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody
    See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
  • Description
    Horse polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay)
  • SpecificityThis antibody is specific for Hepatitis B surface antigen
  • Tested applicationsSuitable for: Dot Blot, ELISA, IHC-Fr, IP, WB, ICC/IFmore details
  • Species reactivity
    This antibody does not react with human serum proteins
  • Immunogen

    Immunization of horses with highly purified Ad/Ay antigen.



Our Abpromise guarantee covers the use of ab9193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/1000. PubMed: 19740987
ICC/IF Use at an assay dependent concentration.


  • RelevanceHepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
  • Cellular localizationVirion membrane
  • Alternative names
    • HBsAg antibody
    • HBV major surface antigen antibody
    • HBV surface antigen antibody
    • Hepatitis B Virus major surface antigen antibody
    • Major surface antigen antibody
    • S antibody
    see all

References for Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (ab9193)

This product has been referenced in:
  • Endmann A  et al. Cationic lipid-formulated DNA vaccine against hepatitis B virus: immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine. PLoS One 9:e101715 (2014). WB . Read more (PubMed: 24992038) »
  • Kwei K  et al. Impaired virion secretion by hepatitis B virus immune escape mutants and its rescue by wild-type envelope proteins or a second-site mutation. J Virol 87:2352-7 (2013). WB ; Hepatitis B virus . Read more (PubMed: 23221548) »

See all 6 Publications for this product

Product Wall

The designation Ad and Ay refer to the subtypes of Hepatitis B Surface Antigen. The reference in the product profile simply means that the antigen used was a mixture of both rather than one or the other.

Unfortunately, I have to be the messenger to confirm that indeed this lot is affected.

Please let me know what kind of compensation your customer would like to receive from us.

Here are a few specific sELISA protocol details I was able to find:

ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL. Read More

If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

We have not yet de...

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Thank you for contacting us.
The antigen was purified from human serum, heat inactivated and injected into horse. The exact immunogen sequence is not known; immunogen contains L, M & S proteins.
I hope this information is helpful to you. Plea...

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Vielen Dank für Ihre Anfrage. Es tut mir leid, dass Sie so lange auf eine Antwort warten mussten. Meine Kollegin Claudia, mit der Sie telefoniert hatten, ist derzeit für Abcam unterwegs und nicht im Büro, hat zuvor jedoch noch genauere Informationen...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Huh7 Human Hepatoma Cells)
Specification Huh7 Human Hepatoma Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Saponin

Abcam user community

Verified customer

Submitted Mar 22 2011

I would recommend a starting dilution of 1:1500 with this product. Currently we do not have an image available with this antibody but we will provide you with one when it becomes available.