Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (Biotin) (ab20778)

Overview

• Product name
  Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (Biotin)
  See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
• Description
  Goat polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay) (Biotin)
• Conjugation
  Biotin
• Specificity
  Non-reactive with normal human serum. Monospecific, reacts only with Hepatitis B virus.
• Tested applications
  Suitable for: ICC/IF, ELISAmore details
• Immunogen

  Mixture of subtypes ad & ay. Hepatitis B surface antigen purified from human serum

• General notes

  Covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.1% Sodium Azide
  Constituents: 10mM PBS, pH 7.2
• Concentration information loading...
• Purity
  IgG fraction
• Purification notes
  Purified IgG fraction of the antiserum covalently coupled with the N-Hydroxysuccinimide ester of Biotin.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Microbiology
  • Organism
  • Virus
  • DNA Virus
  • double stranded DNA Virus
  • Hepatitis B

  • Cancer
  • Oncoproteins/suppressors
  • Viral proteins
  • Hepatitis B

Associated products

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)

Applications

Target

• Relevance
  Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
• Cellular localization
  Virion membrane
• Alternative names
  • HBsAg antibody
  • HBV major surface antigen antibody
  • HBV surface antigen antibody

  • Hepatitis B Virus major surface antigen antibody
  • Major surface antigen antibody
  • S antibody

  see all

Protocols

• Immunocytochemistry & immunofluorescence protocols