Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (Biotin) (ab20778)

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Overview

  • Product name
    Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (Biotin)
    See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
  • Description
    Goat polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay) (Biotin)
  • Host species
    Goat
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ICC/IF, ELISAmore details
  • Immunogen

    Mixture of subtypes ad & ay. Hepatitis B surface antigen purified from human serum

  • General notes

    Covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

Applications

Our Abpromise guarantee covers the use of ab20778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
  • Application notes
    ELISA: 1/1:1000.
    IF: Use at an assay dependent dilution. Use Avidin and streptavidin amplifications systems.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance
      Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
    • Cellular localization
      Virion membrane
    • Alternative names
      • HBsAg antibody
      • HBV major surface antigen antibody
      • HBV surface antigen antibody
      • Hepatitis B Virus major surface antigen antibody
      • Major surface antigen antibody
      • S antibody
      see all

    References

    ab20778 has not yet been referenced specifically in any publications.

    Publishing research using ab20778? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

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    I'd like to buy the Abcam antibody against HBsAg ab20778, and I'd like to know if product ab6741 can be used as a secondary antibody for Western Blot detection, even if the primary antibody is biotin-conjugated. Thank you in advance, waiting for your ...

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    Thank you for your enquiry. I would not recommend to use an HRP-conjugated secondary antibody to use ab20778 in western blotting, as this may not work. The antibody ab20778 has been tested in western blotting using an avidin/streptavidin amplificat...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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