Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted (ab859)
Key features and details
- Mouse monoclonal [3E7] to Hepatitis B Virus Surface Antigen, prediluted
- Suitable for: IHC-P
- Reacts with: Hepatitis B virus
- Isotype: IgG1
Overview
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Product name
Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted
See all Hepatitis B Virus Surface Antigen primary antibodies -
Description
Mouse monoclonal [3E7] to Hepatitis B Virus Surface Antigen, prediluted -
Host species
Mouse -
Specificity
This antibody stains the cytoplasm of antigen-positive liver cells in patients with Type B viral hepatitis. This antibody reacts with the ‘a’ determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Full length protein corresponding to Mouse Hepatitis B Virus Surface Antigen. BALB/c mice were immunized with purified HBsAG subtype AYW3.
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Positive control
- IHC-P: Hepatitis B infected liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Inert stabilizer -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
3E7 -
Myeloma
unknown -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/25 - 1/50.
Pretreat tissue with citrate buffer pH 6. Incubate with primary antibody for 30 minutes at room temperature. |
Notes |
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IHC-P
1/25 - 1/50. Pretreat tissue with citrate buffer pH 6. Incubate with primary antibody for 30 minutes at room temperature. |
Target
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Relevance
Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction. -
Cellular localization
Virion membrane -
Database links
- Entrez Gene: 2703546 Hepatitis B virus
- SwissProt: P03142 Hepatitis B virus
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Alternative names
- Large envelope protein antibody
- Large surface protein antibody
- LHB antibody
- Major surface antigen antibody
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab859 has been referenced in 1 publication.
- He Z et al. Expression of hepatitis B surface antigen in liver tissues can serve as a predictor of prognosis for hepatitis B virus-related hepatocellular carcinoma patients after liver resection. Eur J Gastroenterol Hepatol 33:76-82 (2021). PubMed: 32049678