Anti-Hepatitis B Virus Surface Antigen antibody [HB6] (HRP) (ab2042)

Overview

  • Product name
    Anti-Hepatitis B Virus Surface Antigen antibody [HB6] (HRP)
    See all Hepatitis B Virus Surface Antigen primary antibodies
  • Description
    Mouse monoclonal [HB6] to Hepatitis B Virus Surface Antigen (HRP)
  • Conjugation
    HRP
  • Specificity
    Reacts with the following HbsAg subtypes : ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-, ayw3 (Fer). 
  • Tested applications
    Suitable for: ELISA, Sandwich ELISAmore details
  • Immunogen

    Recombinant full length protein (HBsAg of ayw subtype).

  • General notes
    Concentration varies from lot to lot and can be provided on request.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab2042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [HB12] to Hepatitis B Virus Surface Antigen (ab2039) and Mouse monoclonal [HB5] to Hepatitis B Virus Surface Antigen (ab8333). Can be used as Detection antibody.

Target

  • Relevance
    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
  • Cellular localization
    Virion membrane
  • Alternative names
    • Large envelope protein antibody
    • Large surface protein antibody
    • LHB antibody
    • Major surface antigen antibody

References

ab2042 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Here are a few specific sELISA protocol details I was able to find:

ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL. Read More



If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

We have not yet de...

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Thank you for your enquiry. I would just like to bring to your attention the fact that ab2039 has a matched pair for ELISA's. If you would like to use ab2039 as the coating antibody, the detection pair for that antibody is ab2042. Ab2042 is an HRP conj...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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