Anti-Hepatitis B Virus Surface Antigen antibody [S 26] (ab18797)

Overview

  • Product nameAnti-Hepatitis B Virus Surface Antigen antibody [S 26]
    See all Hepatitis B Virus Surface Antigen primary antibodies
  • Description
    Mouse monoclonal [S 26] to Hepatitis B Virus Surface Antigen
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    HBsAg pre-S antigen.
  • Immunogen

    Full length native protein (purified).

  • EpitopeAmino acids 132-137 of pre-S.

Properties

Applications

Our Abpromise guarantee covers the use of ab18797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.
    Immunofluorescent and immunoperoxidase staining of infected liver tissues and HBV-transfected hepatoma cells, immunoelectron microscopy.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
    • Cellular localizationVirion membrane
    • Alternative names
      • Large envelope protein antibody
      • Large surface protein antibody
      • LHB antibody
      • Major surface antigen antibody

    References for Anti-Hepatitis B Virus Surface Antigen antibody [S 26] (ab18797)

    ab18797 has not yet been referenced specifically in any publications.

    Product Wall

    Virtually all anti-HBsAg mabs (including this one) are conformation-specific, i.e., they recognize only native antigen. That's why they do not work usually neither in WB nor in denaturing ELISA. ab18797 will work in both of those assays, but only the...

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    I have received feedback from the laboratory saying "Your customer is doing everything right, the dilution is 1 to 1000 and 5% dry milk for 1 hour blocking is what we recommend. At this point we can only assume that something happened to product in tra...

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    Thank you very much for contacting us. I just wanted to let you know that I am still working on your enquiry. I have contacted the originator of this antibody for the information you requested, and am awaiting a reply. I'm very sorry for the del...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"