Hepatitis B Virus Surface Antigen (Ayw) full length protein (ab113610)

Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • AccessionQ9PX80
    • Molecular weight25 kDa
    • Amino acids1 to 226
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab113610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab113610 reacts with Human HBV positive serum
  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Purity> 90 % by SDS-PAGE.
    ab113610 is purified by Nickel affinity and Ion Exchange chromatography
  • FormLiquid
  • Additional Notesab113610 reacts with Human HBV positive serum
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.20
    Constituents: 0.1% Phosphate Buffer, 0.44% Sodium chloride, 50% Glycerol

General info

  • Alternative names
    • HBsAg
    • Hep B surface antigen
    • Hepatitis B Virus major surface antigen
    • Hepatitis B virus S antigen
    • Large envelope protein
    • Large surface protein
    • LHB
    • Major surface antigen
    see all
  • RelevanceHepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
  • Cellular localizationCytoplasmic

References for Hepatitis B Virus Surface Antigen (Ayw) full length protein (ab113610)

ab113610 has not yet been referenced specifically in any publications.

Product Wall



Per the lab, this product is an aggregated protein that without heating would run as monomers, dimers and multimers on Western Blots and in SDS-PAGE.

Per the lab, the antigen assembles into particles and they recommend sonication, but please be advised, they have not performed this.

Here are a few specific sELISA protocol details I was able to find:

ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL. Read More



If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

We have not yet de...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"