Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)

Overview

  • Product name
    Anti-Hepatitis B Virus X antigen antibody [3F6-G10]
    See all Hepatitis B Virus X antigen primary antibodies
  • Description
    Mouse monoclonal [3F6-G10] to Hepatitis B Virus X antigen
  • Specificity
    HB-X antigen (17 kD). We have received mixed reports regarding how ab235 works in Western blot, and we are not able to guarantee this antibody in WB.
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Frmore details
  • Immunogen

    HB-Xag-Protein A Fusion protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    3F6-G10
  • Myeloma
    unknown
  • Isotype
    IgG2a
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/100. This antibody may not require protein digestion pre-treatment or antigen retrieval on paraffin sections.
IHC-Fr 1/100.

Target

  • Relevance
    Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.
  • Alternative names
    • HBV X antigen antibody
    • HBx antibody
    • HBxAg antibody

References for Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)

This product has been referenced in:
  • Yen CJ  et al. Hepatitis B Virus X Protein Upregulates mTOR Signaling through IKKß to Increase Cell Proliferation and VEGF Production in Hepatocellular Carcinoma. PLoS One 7:e41931 (2012). Read more (PubMed: 22848663) »
  • Fernandez AF  et al. The dynamic DNA methylomes of double-stranded DNA viruses associated with human cancer. Genome Res 19:438-51 (2009). WB . Read more (PubMed: 19208682) »

See all 4 Publications for this product

Product Wall

Sorry for the delay in getting back to you. I now have further information to share with you.

I have checked into whether anything is know about the epitope of the anti-Hepatitis B Virus X antigen antibodies ab235, ab2741 and ab39716. I can o...

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Thank you for contacting us and your interest in our products.

The request for a quote for the antibodies mentioned has been passed on to one of my colleagues who will get back to you shortly with this information. In regards to your other qu...

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Thank you for contacting us. The note on the datasheet is from the originator of the 3F6-G10 clone. Production of the clone and testing against the recombinant protein is described in the following paper: Pál J et al. Immunohistochemical assessment ...

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Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7 cells)
Loading amount
20 µg
Specification
huh-7 cells
Treatment
Cells are expressing recombinant HBX protein
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 22 2009

Thank you for your message. Unfortuantley, I am unable to obtain precise information about which subtypes of Hepatitis B virus these antibodies detect. We aim to provide as much information as possible to customers, and I am sorry this is not possib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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