Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)
Key features and details
- Mouse monoclonal [3F6-G10] to Hepatitis B Virus X antigen
- Suitable for: ELISA, IHC-P, IHC-Fr
- Reacts with: Hepatitis B virus
- Isotype: IgG2a
Overview
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Product name
Anti-Hepatitis B Virus X antigen antibody [3F6-G10]
See all Hepatitis B Virus X antigen primary antibodies -
Description
Mouse monoclonal [3F6-G10] to Hepatitis B Virus X antigen -
Host species
Mouse -
Specificity
HB-X antigen (17 kD). We have received mixed reports regarding how ab235 works in Western blot, and we are not able to guarantee this antibody in WB. -
Tested applications
Suitable for: ELISA, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Fusion protein corresponding to Hepatitis B Virus X antigen. HB-Xag-Protein A Fusion protein. Spleen cells from immunised BALB/c mice were fused with cells of the Sp-2/0-Ag14 mouse myeloma cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3F6-G10 -
Myeloma
unknown -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
1/100.
This antibody may not require protein digestion pre-treatment or antigen retrieval on paraffin sections. |
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IHC-Fr |
1/100.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
1/100. This antibody may not require protein digestion pre-treatment or antigen retrieval on paraffin sections. |
IHC-Fr
1/100. |
Target
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Relevance
Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC. -
Database links
- SwissProt: O91531 Hepatitis B virus
- SwissProt: Q4R1S1 Hepatitis B virus
- SwissProt: Q91C38 Hepatitis B virus
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Alternative names
- HBV X antigen antibody
- HBx antibody
- HBxAg antibody
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab235 has been referenced in 14 publications.
- Guan H et al. DNA methyltransferase 1 knockdown reverses PTEN and VDR by mediating demethylation of promoter and protects against renal injuries in hepatitis B virus-associated glomerulonephritis. Cell Biosci 12:98 (2022). PubMed: 35765066
- Wan T et al. NEDD4 Induces K48-Linked Degradative Ubiquitination of Hepatitis B Virus X Protein and Inhibits HBV-Associated HCC Progression. Front Oncol 11:625169 (2021). PubMed: 33767993
- Hamamoto S et al. Enhancement of chondrogenic differentiation supplemented by a novel small compound for chondrocyte-based tissue engineering. J Exp Orthop 7:10 (2020). PubMed: 32146609
- Xie WH et al. Hepatitis B virus X protein promotes liver cell pyroptosis under oxidative stress through NLRP3 inflammasome activation. Inflamm Res 69:683-696 (2020). PubMed: 32347316
- Song C et al. Associations Between Hepatitis B Virus Infection and Risk of All Cancer Types. JAMA Netw Open 2:e195718 (2019). PubMed: 31199446