Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)

Overview

  • Product nameAnti-Hepatitis B Virus X antigen antibody [X36C]
    See all Hepatitis B Virus X antigen primary antibodies
  • Description
    Mouse monoclonal [X36C] to Hepatitis B Virus X antigen
  • Tested applicationsSuitable for: IHC-P, ELISA, IP, WBmore details
  • Species reactivity
    Reacts with: Hepatitis B virus
  • Immunogen

    Recombinant full length protein corresponding to Hepatitis B Virus X antigen.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesHepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein-protein interaction.
  • ClonalityMonoclonal
  • Clone numberX36C
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml.

Target

  • RelevanceHepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.
  • Database links
    • Alternative names
      • HBV X antigen antibody
      • HBx antibody
      • HBxAg antibody

    Anti-Hepatitis B Virus X antigen antibody [X36C] images

    • All lanes : Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741) at 1/2000 dilution

      Lane 1 : MIHA hepatocytes
      Lane 2 : MIHA hepatocytes infected with lentivirus containing EGFP alone
      Lane 3 : MIHA hepatocytes infected with lentivirus containing full length Hepatitis B Virus X antigen
      Lane 4 : MIHA hepatocytes infected with lentivirus containing truncated Hepatitis B Virus X antigen
      Lane 5 : MIHA hepatocytes infected with lentivirus containing truncated Hepatitis B Virus X antigen

      Image from Yip WK et al., PLoS One. 2011;6(8):e22888. Epub 2011 Aug 4. Fig 2.; doi:10.1371/journal.pone.0022888; August 4, 2011, PLoS ONE 6(8): e22888.

    References for Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)

    This product has been referenced in:
    • Yip WK  et al. Carboxyl-terminal truncated HBx regulates a distinct microRNA transcription program in hepatocellular carcinoma development. PLoS One 6:e22888 (2011). WB . Read more (PubMed: 21829663) »

    See 1 Publication for this product

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    Thank you for your message. Unfortuantley, I am unable to obtain precise information about which subtypes of Hepatitis B virus these antibodies detect. We aim to provide as much information as possible to customers, and I am sorry this is not possib...

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    The immunogen used was the whole recombinant Hepatitis B X protein and the epitope has not been determined.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"