Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)
Key features and details
- Biotin Mouse monoclonal [NS5B-6] to Hepatitis C Virus
- Suitable for: Flow Cyt (Intra), WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-Hepatitis C Virus antibody [NS5B-6]
See all Hepatitis C Virus primary antibodies -
Description
Biotin Mouse monoclonal [NS5B-6] to Hepatitis C Virus -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli
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Positive control
- Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
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General notes
Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
filter-sterilized -
Clonality
Monoclonal -
Clone number
NS5B-6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa).
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa). |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. -
Cellular localization
Virus surface -
Database links
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P27958 Hepatitis C virus
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Alternative names
- Genome polyprotein antibody
- HCV antibody
- Hepatitis C virus polyprotein antibody
Images
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Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972) + Lysate of chimp liver cells infected with recombinant
vaccinia virus containing a HCV genome cDNA
Predicted band size: 70 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
NS5b protein is detected as a 58kDa band. -
ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab122972 has been referenced in 3 publications.
- Zhang MQ et al. A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells. Autophagy 16:2167-2179 (2020). PubMed: 31996071
- Zhang MQ et al. Differential Effects of Autophagy-Related 10 Protein on HCV Replication and Autophagy Flux Are Mediated by Its Cysteine44 and Cysteine135. Front Immunol 9:2176 (2018). PubMed: 30319633
- Manabe S et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). PubMed: 8291245