Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972)

Overview

  • Product name
    Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin)
    See all Hepatitis C Virus primary antibodies
  • Description
    Mouse monoclonal [NS5B-6] to Hepatitis C Virus (Biotin)
  • Conjugation
    Biotin
  • Specificity
    ab122972 recognizes NS5b (also called RNA-directed RNA polymerase).
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli

  • Positive control
    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
  • General notes

    Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    filter-sterilized
  • Clonality
    Monoclonal
  • Clone number
    NS5B-6
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
  • Cellular localization
    Virus surface
  • Database links
    • Alternative names
      • Genome polyprotein antibody
      • HCV antibody
      • Hepatitis C virus polyprotein antibody

    Images

    • Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972) + Lysate of chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA

      Predicted band size : 70 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.

    References

    This product has been referenced in:
    • Manabe S  et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). Read more (PubMed: 8291245) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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