Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972)


  • Product nameAnti-Hepatitis C Virus antibody [NS5B-6] (Biotin)
    See all Hepatitis C Virus primary antibodies
  • Description
    Mouse monoclonal [NS5B-6] to Hepatitis C Virus (Biotin)
  • ConjugationBiotin
  • Specificityab122972 recognizes NS5b (also called RNA-directed RNA polymerase).
  • Tested applicationsSuitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Hepatitis C Virus
  • Immunogen

    Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli

  • Positive control
    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
  • General notes

    Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesfilter-sterilized
  • ClonalityMonoclonal
  • Clone numberNS5B-6
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab122972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.


  • RelevanceHepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
  • Cellular localizationVirus surface
  • Database links
    • Alternative names
      • Genome polyprotein antibody
      • HCV antibody
      • Hepatitis C virus polyprotein antibody

    Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) images

    • Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972) + Lysate of chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA

      Predicted band size : 70 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.

    References for Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972)

    This product has been referenced in:
    • Manabe S  et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). Read more (PubMed: 8291245) »

    See 1 Publication for this product

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