Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740)


  • Product nameAnti-Hepatitis C Virus Core 1b antibody [C7-50]
  • Description
    Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
  • SpecificityDetects hepatitis C virus (HCV) core protein from transfected human and primate cell lines.
  • Tested applicationsSuitable for: IP, Flow Cyt, ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Hepatitis C Virus
  • Immunogen

    Recombinant full length protein (GST-tag) corresponding to Hepatitis C Virus Core 1b.

  • EpitopeThis antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains.



Our Abpromise guarantee covers the use of ab2740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 20422251
IHC-P Use at an assay dependent concentration. PubMed: 25155355
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.


  • RelevanceHCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression. There are over 20 different subtypes of Hepatitis C Virus; HCV type 1b is mostly found in Europe and Asia. The prevalence of HCV type 1b infection has recently decreased, although it still accounts for most HCV-related cirrhosis and hepatocellular carcinoma. High HCV viremia levels and HCV genotype type 1b are independent predictors for poor response to interferon-alpha therapy. HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine.
  • Cellular localizationSecreted; Host endoplasmic reticulum membrane; Single-pass membrane protein; Host nucleus; Host cytoplasm.
  • Database links
    • Alternative names
      • Capsid protein C antibody
      • Core protein p19 antibody
      • Core protein p21 antibody
      • Hepatitis C virus core protein antibody

    Anti-Hepatitis C Virus Core 1b antibody [C7-50] images

    • ab2740 staining Hepatitis C Virus Core Antigen in the Huh7-Lunet Cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 37°C. An undiluted Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal was used as the secondary antibody.

      See Abreview

    • Immunofluorescence analysis of Huh-7.5.1 cells infected with either Hepatitis C virus (J6/JFH-C) or Hepatitis C virus defective in RNA polymerase activity (J6/JFH pol-null).
      Hepatitis C Virus Core Antigen (red) was stained with ab2740 at 1/300 dilution, 96 hours after infection. A Cy3®-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    References for Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740)

    This product has been referenced in:
    • Xue J  et al. MEAN inhibits hepatitis C virus replication by interfering with a polypyrimidine tract-binding protein. J Cell Mol Med 20:1255-65 (2016). WB, ICC/IF . Read more (PubMed: 26929148) »
    • Yao W  et al. Endoplasmic reticulum stress links hepatitis C virus RNA replication to wild-type PGC-1a/liver-specific PGC-1a upregulation. J Virol 88:8361-74 (2014). Read more (PubMed: 24829353) »

    See all 22 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (human hepatocarcinoma)
    Antigen retrieval step None
    Permeabilization Yes - H2O2
    Specification human hepatocarcinoma
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.05% · Temperature: 24°C
    Fixative Methanol


    Verified customer

    Submitted Jul 01 2016

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (12%)
    Sample Human Cell lysate - whole cell (human liver carcinoma cell line)
    Specification human liver carcinoma cell line
    Treatment transfection of Core protein expressed vector
    Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Oct 29 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Huh7-Lunet Cells)
    Specification Huh7-Lunet Cells
    Fixative Paraformaldehyde
    Permeabilization Yes - Triton X-100
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Feb 19 2013

    Thank you for confirming the details of this product.

    I am pleased to provide thetesting protocol for ELISA using this antibody which I have copied below. Please note, this is a guideline only and may require some individual optimization. Read More

    It is not known. From epitope mapping it has been shown to be directed against aa 21-40 of the HCV core region. I would suggest seeing how conserved this sequence is in 1a.