Anti-Hepatitis C Virus Core Antigen antibody [11-B3] (ab18929)

Overview

  • Product nameAnti-Hepatitis C Virus Core Antigen antibody [11-B3]
    See all Hepatitis C Virus Core Antigen primary antibodies
  • Description
    Mouse monoclonal [11-B3] to Hepatitis C Virus Core Antigen
  • Tested applicationsSuitable for: IHC-Fr, ELISA, WBmore details
  • Species reactivity
    HCV core antigen.
  • Immunogen

    Recombinant full length HCV core from 1B strain.

  • EpitopeAmino acids 70-90.
  • General notesMouse monoclonal [11-B3] to Hepatitis C Virus Core Antigen (ab18929) stains primarily cytoplasmic; mainly in the perinuclear area.

Properties

Applications

Our Abpromise guarantee covers the use of ab18929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/20.
ELISA Use at an assay dependent concentration.
WB 1/1000.

Target

  • RelevanceThe hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.
  • Cellular localizationEndoplasmic reticulum
  • Alternative names
    • Core protein p21 antibody
    • HCV core antigen antibody
    • HCV core protein antibody
    • Hepatitis C Virus core protein antibody

References for Anti-Hepatitis C Virus Core Antigen antibody [11-B3] (ab18929)

ab18929 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (hepatoma cell lline)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Treatment transfection of HCV core plasmid
Specification hepatoma cell lline
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"