Anti-Hepatitis C Virus Core Antigen antibody [11-B3] (ab18929)

Overview

  • Product name
    Anti-Hepatitis C Virus Core Antigen antibody [11-B3]
    See all Hepatitis C Virus Core Antigen primary antibodies
  • Description
    Mouse monoclonal [11-B3] to Hepatitis C Virus Core Antigen
  • Tested applications
    Suitable for: IHC-Fr, ELISA, WBmore details
  • Species reactivity
    HCV core antigen.
  • Immunogen

    Recombinant full length HCV core from 1B strain.

  • Epitope
    Amino acids 70-90.
  • General notes
    Mouse monoclonal [11-B3] to Hepatitis C Virus Core Antigen (ab18929) stains primarily cytoplasmic; mainly in the perinuclear area.

Properties

Applications

Our Abpromise guarantee covers the use of ab18929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/20.
ELISA Use at an assay dependent concentration.
WB 1/1000.

Target

  • Relevance
    The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.
  • Cellular localization
    Endoplasmic reticulum
  • Alternative names
    • Core protein p21 antibody
    • HCV core antigen antibody
    • HCV core protein antibody
    • Hepatitis C Virus core protein antibody

References

ab18929 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (hepatoma cell lline)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Treatment
transfection of HCV core plasmid
Specification
hepatoma cell lline
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Submitted May 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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