Anti-Hepatitis C Virus Core Antigen antibody [1F6] (ab2586)

Overview

  • Product nameAnti-Hepatitis C Virus Core Antigen antibody [1F6]
    See all Hepatitis C Virus Core Antigen primary antibodies
  • Description
    Mouse monoclonal [1F6] to Hepatitis C Virus Core Antigen
  • SpecificityThis antibody is specific for Hepatitis C Core Antigen.
  • Tested applicationsSuitable for: IP, ELISAmore details
    Unsuitable for: WB
  • Immunogen

    Recombinant fragment.

  • EpitopeThis antibody recognises amino acid residues 80-120 of Hepatitis C Core Antigen.

Properties

Applications

Our Abpromise guarantee covers the use of ab2586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceThe hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.
    • Cellular localizationEndoplasmic reticulum
    • Alternative names
      • Core protein p21 antibody
      • HCV core antigen antibody
      • HCV core protein antibody
      • Hepatitis C Virus core protein antibody

    References for Anti-Hepatitis C Virus Core Antigen antibody [1F6] (ab2586)

    ab2586 has not yet been referenced specifically in any publications.

    Product Wall

    Application ELISA
    Sample Hepatitis C Virus Purified protein (E.coli)
    Specification E.coli
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Type Sandwich (Capture)
    Username

    Abcam user community

    Verified customer

    Submitted Jun 02 2016

    ab2586 is reactive with genotype 1b, not 2A.

    Thank you for your enquiry. The HCV anti core, ab2586, was made to a 1B genotype. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for your enquiry. I apologize, but it seems there was an error on the ab2586 datasheet. The antibody was tested in Western blot and found unsuitable. I have updated the datasheet with this information to avoid future confusion. I hope...

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    Thank you for your reply. Yes, core was IPed out of cells expressing core protein. I apologize that the recipe was not included in the last email. Please find the recipe below: NP-40 Cell Lysis Buffer - Immunoprecipitation Buffer Recipe ...

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    I am sorry for the long delay with answering this question, the supplier has only just gotten back to me. The HCV core antigen is 1B genotype.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"