• Product nameAnti-Hepatitis C Virus E2 antibody
    See all Hepatitis C Virus E2 primary antibodies
  • Description
    Goat polyclonal to Hepatitis C Virus E2
  • SpecificityE2 envelope protein of Hepatitis C Virus.
  • Tested applicationsSuitable for: ELISA, WB, Conjugationmore details
  • Species reactivity
    Reacts with Hepatitis C Virus. Not yet tested in other species.
  • Immunogen

    Recombinant full length E2 (genotype 1a) protein.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notes>95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab20044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.
Conjugation Use at an assay dependent dilution.


  • RelevanceHepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.
  • Cellular localizationViral envelope protein.
  • Alternative names
    • Core protein antibody
    • E1 protein antibody
    • E2 protein antibody
    • Envelope glycoprotein E2 antibody
    • gp68 antibody
    • gp70 antibody
    • HCV E2 antibody
    • NS1 antibody
    • NS2 protein antibody
    • NS3 protease/helicase antibody
    • NS4A protein antibody
    • NS4B protein antibody
    • NS5A protein antibody
    • NS5B RNA dependent RNA polymerase antibody
    • P7 protein antibody
    • Polyprotein antibody
    see all

Anti-Hepatitis C Virus E2 antibody images

  • ELISA analysis of Hepatitis C Virus E2 binding to CD81 receptor, using ab20044 to detect Hepatitis C Virus E2 binding.

    A 96-well plate was coated with 100 ng of rho-1D4 antibody. Following overnight incubation at 4°C, the unbound antibody was washed away. The wells were blocked with blocking solution for 2 hours. 100 ng of purified CD81 was added to the wells along with E2 glycoprotein (0.4 to 20 nM) and incubated for 2 hours. ab20044 anti-Hepatitis C Virus E2 antibody (1/2000 dilution) and secondary antibody with alkaline phosphatase conjugate (1/1000 dilution) were added and incubated for 2 hours. Absorbance was measured at 495 nm by a plate-reader.

References for Anti-Hepatitis C Virus E2 antibody (ab20044)

This product has been referenced in:
  • Takayama H  et al. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3:e2314 (2008). ELISA ; Hepatitis C Virus . Read more (PubMed: 18523555) »

See 1 Publication for this product

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ab20044 and ab20852 have not been tested in neutralisation experiment. We have no empirical data to support theuse in the application.

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Following your enquiry I have checked all our HAV, HCV and Parvovirus B19 antibodies and doublechecked with the originators of these if the antibodies would detect intact virions. Thank you for your patience whil...

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Thank you for your enquiry. I can confrim that the immunogen for ab20044 Hepatitis C Virus E2 antibody is a recombinant antigen expressed in CHO cells. This is a polyclonal antibody and therefore recognizes multiple epitopes on the E2 protein, but...

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