Anti-Hepatitis C Virus genotype 1a NS5 antibody (ab20773)

Overview

  • Product name
    Anti-Hepatitis C Virus genotype 1a NS5 antibody
  • Description
    Goat polyclonal to Hepatitis C Virus genotype 1a NS5
  • Specificity
    Ab20773 specifically targets NS5a + NS5b of Hepatitis C Virus.
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with Hepatitis C Virus. Not yet tested in other species.
  • Immunogen

    Recombinant NS5 a&b genotype 1a (100% of a and 70% of b).

Properties

Applications

Our Abpromise guarantee covers the use of ab20773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. WB: 1/100. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008). NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication. Hepatitis C virus (HCV) can cause chronic hepatitis, cirrhosis and hepatocellular carcinoma. At present there is no vaccine effective against HCV.
    • Cellular localization
      Endoplasmic reticulum membrane
    • Alternative names
      • HCV 1a NS5 antibody
      • Hepatitis C virus NS5A antibody
      • Non-structural protein 5A antibody
      • p56 antibody

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Thanks for sending along your protocol.

    1. ab49033 is unlikely to be detected by these antibodies as it only contains about 100 aa of the total protein. As described on datasheet this product containsamino acids"corresponding to amino ac...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Cell (huh 7 cells replicon)
    Specification
    huh 7 cells replicon
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
    Type
    Sandwich (Capture)
    Username

    Abcam user community

    Verified customer

    Submitted Jul 30 2008

    Thank you for your enquiry. I looked up some Hepatitis C Virus antibodies that may be of interest to you and the information is listed below. It does not appear that we have one that is specific for strain 2a or has even bee tested for reactivity with ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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