Anti-Hepatitis C Virus NS3 antibody [8 G-2] (ab65407)

Overview

  • Product nameAnti-Hepatitis C Virus NS3 antibody [8 G-2]
    See all Hepatitis C Virus NS3 primary antibodies
  • Description
    Mouse monoclonal [8 G-2] to Hepatitis C Virus NS3
  • SpecificityReacts efficiently with JFH-1 strain of HCV (genotype 2a)
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Hepatitis C Virus
  • Immunogen

    recombinant NS3 protein

  • Epitopeamino acids 1340 – 1470 of HCV polyprotein
  • Positive control
    • Huh7 cells harbouring HCV replicon (genotype 1b), Huh7 cells infected with HCV strain JFH-1 (genotype 2a)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mM PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityMonoclonal
  • Clone number8 G-2
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF 1/500.

Target

  • RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localizationEndoplasmic reticulum membrane
  • Database links
    • Alternative names
      • Hepatitis C Virus NS3 antibody
      • NS3 antibody
      • NS3P antibody
      • p70 antibody
      • Serine protease/NTPase/helicase antibody
      see all

    Anti-Hepatitis C Virus NS3 antibody [8 G-2] images

    • ab65407 at 1/500 dilution staining Huh7 cells harbouring HCV replicon (genotype 1b) (left) and Huh7 cells (right). Nuclei were visualised with DAPI (shown in blue).
    • ab65407 at 1/500 dilution staining Huh7 cells infected with HCV strain JFH-1 (left) and mock infected cells (right). Nuclei were visualised with DAPI (shown in green).
    • Immunofluorescence analysis of Hepatitis C infected Human fetal astrocytes, staining Hepatitis C Virus NS3 with ab65407. An AlexaFlour®488-conjugated anti-mouse IgG was used as the secondary antibody.

    References for Anti-Hepatitis C Virus NS3 antibody [8 G-2] (ab65407)

    This product has been referenced in:
    • Wong MT & Chen SS Human Choline Kinase-a Promotes Hepatitis C Virus RNA Replication through Modulation of Membranous Viral Replication Complex Formation. J Virol 90:9075-95 (2016). Read more (PubMed: 27489281) »
    • Zhao Y  et al. Ficolin-2 inhibits hepatitis C virus infection, whereas apolipoprotein E3 mediates viral immune escape. J Immunol 193:783-96 (2014). Read more (PubMed: 24928988) »

    See all 10 Publications for this product

    Product Wall

    Thank you for your reply.

    I have been in contact with the lab and they are not be able to find Western blot image for ab65407 at this point in time.

    I am sorry about that, but please let me know if there is anything else I can help ...

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    Thank you for contacting Abcam.

    I have been in contact with the lab and they have told me that a band of 70-72 kDa is usually detected; often minor additional bands of higher MW are visible, representing incomplete processing of viral polypro...

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    Thank you for contacting us. Unfortunately we currently don't have any FITC conjugated anti-HCV NS3 in our catalog. However, I woud suggest to conjugate the mouse monoclonal Anti-Hepatitis C Virus NS3 antibody [8 G-2] (ab65407) using the...

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    Thank you for your enquiry. I am sorry it has taken some time to request the immunogen seuqences from our sources and review these for you, thank you for your patience. I can confirm the following mouse Hepatitis C Virus NS3 antibodies have been tes...

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