Overview

  • Product name
    Anti-Hepatitis C Virus NS3 antibody [806]
    See all Hepatitis C Virus NS3 primary antibodies
  • Description
    Mouse monoclonal [806] to Hepatitis C Virus NS3
  • Specificity
    ab 18671 reacts with recombinant NS-3 (amino acids 1252-1477), synthetic NS-3 (amino acids 1378-1458), and recombinant chimeric HCV polyprotein (60 kDa). No cross reaction can be seen with recombinant CPC* + EPM** (core) (amino acids 1-142), synthetic CPC* (amino acids 1-61), and synthetic NS-4a protein (amino acids 1689-1735).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    Purified Recombinant Chimeric HCV Polyprotein (555 a.a)

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    806
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 60 kDa.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
    ab18671 at a concentration of 0.1-1 ug/mL will allow visualization of 100 ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 protein.
  • Target

    • Relevance
      HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
    • Cellular localization
      Endoplasmic reticulum membrane
    • Alternative names
      • Hepatitis C Virus NS3 antibody
      • NS3 antibody
      • NS3P antibody
      • p70 antibody
      • Serine protease/NTPase/helicase antibody
      see all

    References

    This product has been referenced in:
    • Stindt S  et al. Hepatitis C Virus Activates a Neuregulin-Driven Circuit to Modify Surface Expression of Growth Factor Receptors of the ErbB Family. PLoS One 11:e0148711 (2016). Read more (PubMed: 26886748) »
    • Kong L  et al. HIV infection of hepatocytes results in a modest increase in hepatitis C virus expression in vitro. PLoS One 9:e83728 (2014). WB . Read more (PubMed: 24586227) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab18671.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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