Overview

  • Product nameAnti-Hepatitis C Virus NS3 antibody [806]
    See all Hepatitis C Virus NS3 primary antibodies
  • Description
    Mouse monoclonal [806] to Hepatitis C Virus NS3
  • Specificityab 18671 reacts with recombinant NS-3 (amino acids 1252-1477), synthetic NS-3 (amino acids 1378-1458), and recombinant chimeric HCV polyprotein (60 kDa). No cross reaction can be seen with recombinant CPC* + EPM** (core) (amino acids 1-142), synthetic CPC* (amino acids 1-61), and synthetic NS-4a protein (amino acids 1689-1735).
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    Purified Recombinant Chimeric HCV Polyprotein (555 a.a)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number806
  • MyelomaSp2/0-Ag14
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 60 kDa.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
    ab18671 at a concentration of 0.1-1 ug/mL will allow visualization of 100 ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 protein.
  • Target

    • RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
    • Cellular localizationEndoplasmic reticulum membrane
    • Alternative names
      • Hepatitis C Virus NS3 antibody
      • NS3 antibody
      • NS3P antibody
      • p70 antibody
      • Serine protease/NTPase/helicase antibody
      see all

    References for Anti-Hepatitis C Virus NS3 antibody [806] (ab18671)

    This product has been referenced in:
    • Kong L  et al. HIV infection of hepatocytes results in a modest increase in hepatitis C virus expression in vitro. PLoS One 9:e83728 (2014). WB . Read more (PubMed: 24586227) »
    • Brenndörfer ED  et al. Nonstructural 3/4A protease of hepatitis C virus activates epithelial growth factor-induced signal transduction by cleavage of the T-cell protein tyrosine phosphatase. Hepatology 49:1810-20 (2009). WB . Read more (PubMed: 19475692) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"