Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)

Overview

  • Product name
    Anti-Hepatitis C Virus NS3 antibody [H23]
    See all Hepatitis C Virus NS3 primary antibodies
  • Description
    Mouse monoclonal [H23] to Hepatitis C Virus NS3
  • Specificity
    Hepatitis C Virus antigen NS3 helicase domain. Reacts very well with JFH-1 strain (genotype 2a)
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Immunogen

    Recombinant full length protein (E. coli).

  • Epitope
    Helicase domain

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    H23
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/1000.
IHC-P 1/50.

Tested using paraffin-embedded liver sections from HCV patients.

Target

  • Relevance
    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localization
    Endoplasmic reticulum membrane
  • Alternative names
    • Hepatitis C Virus NS3 antibody
    • NS3 antibody
    • NS3P antibody
    • p70 antibody
    • Serine protease/NTPase/helicase antibody
    see all

Anti-Hepatitis C Virus NS3 antibody [H23] images

  • ab13830 used in IHC at 1/50 dilution. Cell line used is Huh7 human hepatoma.
  • The negative control for above picture with Huh7 cells.
  • Image from Kota S et al., PLoS One. 2012;7(2):e32207. Epub 2012 Feb 28.; Fig 3.; doi:10.1371/journal.pone.0032207; February 28, 2012, PLoS ONE 7(2): e32207.

    Representative Western Blot detecting Hepatitis C Virus NS3 with ab13830.

    SL209-biotin was immobilized on streptavidin agarose beads and incubated with lysates of Huh-7.5 cells infected with HCV J6/JFH-1. Retained proteins were examined by western blot.

References for Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)

This product has been referenced in:
  • Hansen MD  et al. Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase M. Proc Natl Acad Sci U S A 114:E3462-E3471 (2017). Read more (PubMed: 28389568) »
  • Li X  et al. Hepatocyte nuclear factor 4a and downstream secreted phospholipase A2 GXIIB regulate production of infectious hepatitis C virus. J Virol 88:612-27 (2014). Read more (PubMed: 24173221) »

See all 15 Publications for this product

Product Wall

Thank you for your message.

Reviewing the recommended storage details for these antibodies, I can suggest they should not be significantly affected by short term storage for a week or two at +4oC. However, I would recommend to aliquot and fr...

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Thank you for your enquiry. I am sorry it has taken some time to request the immunogen seuqences from our sources and review these for you, thank you for your patience. I can confirm the following mouse Hepatitis C Virus NS3 antibodies have been tes...

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Thank you for sending me the image of your blot. So, you have no bands (just some background) in the NS3 recombinant protein lane, and the same pattern of bands in the other lanes, including the negative control. Just to make sure, do you know that you...

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Thank you for your message and I'm sorry to hear that you are experiencing difficulty with ab13830. It would be helpful if you could send me an image of the blot. You mention no signal but then mention non-specific bands. How strong are these bands? Wh...

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