Hepatitis C Virus NS3 protein fragment (ab49024)

Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • Amino acids1192 to 1456

Specifications

Our Abpromise guarantee covers the use of ab49024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityStrongly reacts with human HCV positive serum.
  • Applications

    ELISA

    Flow Cytometry

    SDS-PAGE

    Western blot

  • Purity> 95 % by SDS-PAGE.
    Purity of proteins is evaluated by SDS-PAGE
  • FormLiquid
  • Additional NotesStrongly reacts with human HCV positive serum.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 0.2% Triton-X, 1.5M Urea, 25mM Tris HCl, pH 8

General info

  • Alternative names
    • Hepatitis C Virus NS3
    • NS3
    • NS3P
    • p70
    • Serine protease/NTPase/helicase
    see all
  • RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localizationEndoplasmic reticulum membrane

References for Hepatitis C Virus NS3 protein fragment (ab49024)

ab49024 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and your interest in our products. Generally, one vial of 100 ug of protein of these products (ab49022, ab49024, ab49025) typically can give 50 to 100 tests depending on the format. More tests can be performed for ELIS...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"