Anti-Hepatitis C Virus NS4 antibody [5D4/10E7] (ab19048)


  • Product name
    Anti-Hepatitis C Virus NS4 antibody [5D4/10E7]
    See all Hepatitis C Virus NS4 primary antibodies
  • Description
    Mouse monoclonal [5D4/10E7] to Hepatitis C Virus NS4
  • Specificity
    This antibody reacts with synthetic NS4a Protein, and recombinant Chimeric HCV Polyprotein. No cross-reaction with HCV capsid region and other non-structure regions.
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Immunogen

    Synthetic peptide (Human) in which overlapping sequences of at least 90 residues in length were selected from the immunodominant NS4 regions of Chinese HCV strains.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab19048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Immunoperoxidase-avidin-biotin (ABC) assay for formalin-fixed paraffin embedded tissue section using diluted MAb. The dilution of MAb should be tested based on your working system.

WB Use a concentration of 0.5 µg/ml.

Use at a concentration of 0.5 µg/ml to detect 0.5 µg/lane of synthetic NS4 peptide and 0.1 µg/lane recombinant chimeric HCV polyprotein.


  • Relevance
    NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
  • Cellular localization
    Endoplasmic reticulum; multi-pass membrane protein.
  • Alternative names
    • HCV antibody
    • HCV NS4 antibody
    • Hepatitis C Virus nonstructural antigen 4 antibody
    • Non structural protein 4A antibody
    • Non structural protein 4B antibody
    • NS4A antibody
    • NS4B antibody
    • p27 antibody
    • p8 antibody
    see all

Anti-Hepatitis C Virus NS4 antibody [5D4/10E7] images

  • Anti HCV-NS4 MAb Clone 5D4 (ab19048) in liver cancer section.
  • Anti HCV-NS4 MAb Clone 5D4 (ab19048) in liver cancer section.

References for Anti-Hepatitis C Virus NS4 antibody [5D4/10E7] (ab19048)

ab19048 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I was able to receive two IHC pictures of ab19048 from the researcher who tested it in IHC. Please find the two images on the datasheet. The tissue section shown in both was liver cancer section, one picture was at high ...

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