Recombinant Hepatitis C Virus NS4 protein (Biotin) (ab49030)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
Description
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Product name
Recombinant Hepatitis C Virus NS4 protein (Biotin)
See all Hepatitis C Virus NS4 proteins and peptides -
Biological activity
Reacts strongly with human HCV positive serum -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino acids
1658 to 1863
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Conjugation
Biotin
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab49030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Form
Liquid -
Additional notes
Reacts strongly with human HCV positive serum -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: 48% Urea, 0.078% Beta mercaptoethanol, 0.316% Tris HCl
General Info
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Alternative names
- HCV
- HCV NS4
- Hepatitis C Virus nonstructural antigen 4
see all -
Relevance
NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa. -
Cellular localization
Endoplasmic reticulum; multi-pass membrane protein.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab49030 has not yet been referenced specifically in any publications.