Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) (ab124526)

Overview

  • Product name
    Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin)
    See all Hepatitis C Virus NS4A primary antibodies
  • Description
    Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Specific to Human Hepatitis C Virus NS4A protein of genotype 1b. Not tested in other genotypes
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    A region of NS4 protein of Hepatitis C Virus genotype 1b expressed in E. coli.

  • Epitope
    N terminal region of the NS4 protein (NS4A).
  • Positive control
    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
  • General notes

    [biotin] / [IgG] = 11.3

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Clonality
    Monoclonal
  • Clone number
    S4-13
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Hepatitis C Virus is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of Hepatitis C Virus with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha
  • Cellular localization
    Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Host membrane insertion occurs after processing by the NS3 protease.
  • Database links
    • Alternative names
      • core protein antibody
      • E1 protein antibody
      • E2 protein antibody
      • HCV NS4A antibody
      • Non structural protein 4A antibody
      • NS2 protein antibody
      • NS3 protease/helicase antibody
      • NS4A antibody
      • NS4A protein antibody
      • NS4B protein antibody
      • NS5A protein antibody
      • NS5B RNA-dependent RNA polymerase antibody
      • p7 protein antibody
      • polyprotein antibody
      see all

    Images

    • Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) (ab124526) + Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.

      Predicted band size : 6 kDa
    • ab124526 staining Hepatitis C Virus NS4A in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and Hepatitis C Virus NS4A protein was detected by indirect immunofluorescence staining using this antibody.

    References

    ab124526 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting us. In theory the protein ab49029 should react with antibidy ab124526, since the protein is full length Hepatitis C Virus NS4 protein. However, this has not been tested and there is a possibility the tag could prevent the antib...

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    Thank you for contacting us.

    Here is more information on how our tetsing discount peogram works.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:...

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    Thank you for your patience.

    I have good news for you: The unconjugated antibody for ab124526 is now in the catalog and available to be ordered. It is ab128685 (Anti-Hepatitis C Virus NS4A antibody [S4-13]):

    http://www.abcam.com/he...

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    Thank you for contacting us.


    I have the following information for you.

    ab13831: This antibody recognizes proteins of genotypes 1a, 1b, 3 and 4, and possibly others, but not 2a. The following antibody recognizes genotype 2a: An...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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