Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) (ab124526)

Overview

  • Product nameAnti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin)
    See all Hepatitis C Virus NS4A primary antibodies
  • Description
    Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A (Biotin)
  • ConjugationBiotin
  • SpecificitySpecific to Human Hepatitis C Virus NS4A protein of genotype 1b. Not tested in other genotypes
  • Tested applicationsSuitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Hepatitis C Virus
  • Immunogen

    A region of NS4 protein of Hepatitis C Virus genotype 1b expressed in E. coli.

  • EpitopeN terminal region of the NS4 protein (NS4A).
  • Positive control
    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
  • General notes

    [biotin] / [IgG] = 11.3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • ClonalityMonoclonal
  • Clone numberS4-13
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceHepatitis C Virus is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of Hepatitis C Virus with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha
  • Cellular localizationHost endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Host membrane insertion occurs after processing by the NS3 protease.
  • Database links
    • Alternative names
      • core protein antibody
      • E1 protein antibody
      • E2 protein antibody
      • HCV NS4A antibody
      • Non structural protein 4A antibody
      • NS2 protein antibody
      • NS3 protease/helicase antibody
      • NS4A antibody
      • NS4A protein antibody
      • NS4B protein antibody
      • NS5A protein antibody
      • NS5B RNA-dependent RNA polymerase antibody
      • p7 protein antibody
      • polyprotein antibody
      see all

    Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) images

    • Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) (ab124526) + Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.

      Predicted band size : 6 kDa
    • ab124526 staining Hepatitis C Virus NS4A in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and Hepatitis C Virus NS4A protein was detected by indirect immunofluorescence staining using this antibody.

    References for Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) (ab124526)

    ab124526 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. In theory the protein ab49029 should react with antibidy ab124526, since the protein is full length Hepatitis C Virus NS4 protein. However, this has not been tested and there is a possibility the tag could prevent the antib...

    Read More

    Thank you for contacting us.

    Here is more information on how our tetsing discount peogram works.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:...

    Read More

    Thank you for your patience.

    I have good news for you: The unconjugated antibody for ab124526 is now in the catalog and available to be ordered. It is ab128685 (Anti-Hepatitis C Virus NS4A antibody [S4-13]):

    http://www.abcam.com/he...

    Read More

    Thank you for contacting us.


    I have the following information for you.

    ab13831: This antibody recognizes proteins of genotypes 1a, 1b, 3 and 4, and possibly others, but not 2a. The following antibody recognizes genotype 2a: An...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"