Anti-Hepatitis C Virus NS4B antibody [2-H1] (ab24283)


  • Product nameAnti-Hepatitis C Virus NS4B antibody [2-H1]
  • Description
    Mouse monoclonal [2-H1] to Hepatitis C Virus NS4B
  • Specificityab24283 reacts with HCV NS-4B.
  • Tested applicationsSuitable for: WB, IHC-Fr, ICC/IF, ELISAmore details
  • Immunogen

    Recombinant full length protein- HCV genotype 1b.

  • Epitopeab24283 binds Hepatitis C Virus (HCV) NS-4B Region (amino acids. 1710-1730)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number2-H1
  • IsotypeIgG2b
  • Research areas


Our Abpromise guarantee covers the use of ab24283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Dilution optimised using Chromogenic detection.
IHC-Fr 1/20.
ICC/IF Use at an assay dependent concentration. PubMed: 21507970
ELISA 1/1e+006.


  • RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
  • Cellular localizationVirus
  • Alternative names
    • HCV antibody
    • HCV NS4B antibody
    • Non structural protein 4B antibody
    • NS4B antibody
    • p27 antibody
    see all

Anti-Hepatitis C Virus NS4B antibody [2-H1] images

  • ab24283 staining Hepatitis C Virus NS4B in Huh7/T7 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and permeabilized by 0.5% Triton X-100 in PBS for 15 min at room temperature. Samples were incubated with primary antibody (3% BSA in PBS) for 1 hour at room temperature. Nuclei were stained with DAPI.

References for Anti-Hepatitis C Virus NS4B antibody [2-H1] (ab24283)

This product has been referenced in:
  • Wuestenberg A  et al. Matrix Conditions and KLF2-Dependent Induction of Heme Oxygenase-1 Modulate Inhibition of HCV Replication by Fluvastatin. PLoS One 9:e96533 (2014). WB ; Hepatitis C Virus . Read more (PubMed: 24801208) »
  • Gu Z  et al. Identification of PTC725, an orally bioavailable small molecule that selectively targets the hepatitis C Virus NS4B protein. Antimicrob Agents Chemother 57:3250-61 (2013). Read more (PubMed: 23629699) »

See all 6 Publications for this product

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