Anti-Hepatitis C Virus NS4B antibody [2-H1] (ab24283)

Overview

  • Product name
    Anti-Hepatitis C Virus NS4B antibody [2-H1]
  • Description
    Mouse monoclonal [2-H1] to Hepatitis C Virus NS4B
  • Specificity
    ab24283 reacts with HCV NS-4B.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IF, ELISAmore details
  • Immunogen

    Recombinant full length protein- HCV genotype 1b.

  • Epitope
    ab24283 binds Hepatitis C Virus (HCV) NS-4B Region (amino acids. 1710-1730)
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    2-H1
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Dilution optimised using Chromogenic detection.
IHC-Fr 1/20.
ICC/IF Use at an assay dependent concentration. PubMed: 21507970
ELISA 1/1e+006.

Target

  • Relevance
    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
  • Cellular localization
    Virus
  • Alternative names
    • HCV antibody
    • HCV NS4B antibody
    • Non structural protein 4B antibody
    • NS4B antibody
    • p27 antibody
    see all

Anti-Hepatitis C Virus NS4B antibody [2-H1] images

  • ab24283 staining Hepatitis C Virus NS4B in Huh7/T7 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and permeabilized by 0.5% Triton X-100 in PBS for 15 min at room temperature. Samples were incubated with primary antibody (3% BSA in PBS) for 1 hour at room temperature. Nuclei were stained with DAPI.

References for Anti-Hepatitis C Virus NS4B antibody [2-H1] (ab24283)

This product has been referenced in:
  • Jiang XH  et al. Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/ß-catenin pathway. BMC Microbiol 17:124 (2017). Read more (PubMed: 28545480) »
  • Zhu F  et al. A Novel Adeno-Associated Virus-Based Genetic Vaccine Encoding the Hepatitis C Virus NS3/4 Protein Exhibits Immunogenic Properties in Mice Superior to Those of an NS3-Protein-Based Vaccine. PLoS One 10:e0142349 (2015). Read more (PubMed: 26556235) »

See all 9 Publications for this product

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