Anti-Hepatitis C Virus NS5 antibody [BGN/1246/5G7] (ab65296)

Overview

  • Product nameAnti-Hepatitis C Virus NS5 antibody [BGN/1246/5G7]
  • Description
    Mouse monoclonal [BGN/1246/5G7] to Hepatitis C Virus NS5
  • Specificityab65296 detects Hepatitis C Virus NS5 antigen in viral samples.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Hepatitis C Virus
  • Immunogen

    Synthetic peptide (Hepatitis C Virus) corresponding to the NS5 antigen region.

Properties

Applications

Our Abpromise guarantee covers the use of ab65296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
IHC-Fr
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. A major band is obtained at ~50 kDa with minor bands at ~35, ~40, and ~67 kDa. This reflects bands found in DTT reduced Blots of the immunizing antigen and in immunoblots using a known antibody positive serum.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. NS5 (non structural protein 5) may play a role in the viral RNA replication of the Hepatitis C Virus.
    • Database links
      • Alternative names
        • HCV NS5 antibody
        • Hepatitis C virus non structural protein 5 antibody
        • Hepatitis C virus nonstructural protein 5 antibody
        • Hepatitis C Virus NS5 antibody

      References for Anti-Hepatitis C Virus NS5 antibody [BGN/1246/5G7] (ab65296)

      ab65296 has not yet been referenced specifically in any publications.

      Product Wall

      Application Western blot
      Sample Hepatitis C Virus Recombinant protein (Recombinant protein)
      Loading amount 0.25 µg
      Specification Recombinant protein
      Gel Running Conditions Reduced Denaturing (4-12)
      Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
      Username

      Abcam user community

      Verified customer

      Submitted Oct 16 2012

      Thank you for your reply.

      I am sorry that the antibody did not for you as we guaranteed. We are not able to send you a gift card or similar, therefore I have refunded the cost of the original antibody. Your credit note number is******.
      <...

      Read More

      Thank you for your reply.

      I have looked though our catalogue and ab65296, is the only Hepatitis C Virus NS5 antibody that we have. We have several Hepatitis C Virus NS5A or B antibodies, but I am unsure if they would be suitable for your appl...

      Read More

      Thank you for contacting Abcam.

      I am sorry that you are having problems with the NS5 antibody; ab65296 and that you missed your deadline. Under our Abpormise we do guarantee that this antibody will work in WB, ELISA, IHC-P and IHC-Fr, against...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"