Anti-Hepatitis C Virus NS5a antibody [388] (ab20342)

Overview

  • Product name
    Anti-Hepatitis C Virus NS5a antibody [388]
    See all Hepatitis C Virus NS5a primary antibodies
  • Description
    Mouse monoclonal [388] to Hepatitis C Virus NS5a
  • Specificity
    Ab20342 is positive with genotypes 1a and 1b, and is negative with genotype 2a.
  • Tested applications
    Suitable for: IP, ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with HCV. Not yet tested in other species.
  • Immunogen

    Recombinant full length protein (HCV genotype 1a).

  • Epitope
    Epitope has not been mapped.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab13833 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >90% pure
  • Clonality
    Monoclonal
  • Clone number
    388
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 19839005
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Target

  • Relevance
    HCV is classified into the genus Hepacivirus of the family Flaviviridae. Like all the members of the family, HCV is an enveloped, single-stranded, positive-sense RNA virus. Its genome (about 9600 nt) is flanked at both termini by conserved, highly structured non-translated regions (NTRs) and encodes a polyprotein precursor (about 3000 aa), which is proteolytically processed by host and viral proteases to produce the structural (core, E1, E2 and p7) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B) proteins of the virus. Recently, an additional protein has been identified, whose function remains unknown. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008).
  • Alternative names
    • HCV antibody
    • HCV Non-structural protein 5A antibody
    • Non-structural protein 5A antibody
    • NS5A antibody
    • p56 antibody
    see all

Images

  • All lanes : Anti-Hepatitis C Virus NS5a antibody [388] (ab20342) at 1/1000 dilution

    Lane 1 : Huh-7 (negative control)
    Lane 2 : HCV Replicon cell line

    Secondary
    Goat anti-mouse IgG conjugated to HRP

    This image is courtesy of an anonymous Abreview

    See Abreview

References

This product has been referenced in:
  • Govea-Salas M  et al. Gallic acid decreases hepatitis C virus expression through its antioxidant capacity. Exp Ther Med 11:619-624 (2016). Read more (PubMed: 26893656) »
  • Khachatoorian R  et al. A cell-permeable hairpin peptide inhibits hepatitis C viral nonstructural protein 5A-mediated translation and virus production. Hepatology 55:1662-72 (2012). Read more (PubMed: 22183951) »

See all 5 Publications for this product

Customer reviews and Q&As

Thanks for sending along your protocol.

1. ab49033 is unlikely to be detected by these antibodies as it only contains about 100 aa of the total protein. As described on datasheet this product containsamino acids"corresponding to amino ac...

Read More

Thank you for contacting us.

This may sound familiar ;-)

This anti-HCVNS4b and NS5aantibodies are mouse monoclonals of IgG2b andIgG2a isotype, respectively.IgG2 antibodies usually comprise 450 amino acids, resulting in a molecular w...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (huh-7 cells replicon)
Specification
huh-7 cells replicon
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Jul 30 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (liver cell line)
Loading amount
50000 cells
Specification
liver cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

Submitted Jun 28 2006

Thank you for your enquiry. The molecular weight of native HCV NS-5a is a 56kDa. I hope this information helps. Please do not hesitate to contacts us if you need anything further.

Thank you for your enquiry. Ab20342 has not been tested for reactivity to genotype 2a. Also, the epitope has not been determined. Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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