Anti-Hepatitis C Virus NS5a antibody [388] (ab20342)

Overview

  • Product nameAnti-Hepatitis C Virus NS5a antibody [388]
    See all Hepatitis C Virus NS5a primary antibodies
  • Description
    Mouse monoclonal [388] to Hepatitis C Virus NS5a
  • SpecificityAb20342 is positive with genotypes 1a and 1b, and is negative with genotype 2a.
  • Tested applicationsSuitable for: IP, ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with HCV. Not yet tested in other species.
  • Immunogen

    Recombinant full length protein (HCV genotype 1a).

  • EpitopeEpitope has not been mapped.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>90% pure
  • ClonalityMonoclonal
  • Clone number388
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 19839005
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Target

  • RelevanceHCV is classified into the genus Hepacivirus of the family Flaviviridae. Like all the members of the family, HCV is an enveloped, single-stranded, positive-sense RNA virus. Its genome (about 9600 nt) is flanked at both termini by conserved, highly structured non-translated regions (NTRs) and encodes a polyprotein precursor (about 3000 aa), which is proteolytically processed by host and viral proteases to produce the structural (core, E1, E2 and p7) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B) proteins of the virus. Recently, an additional protein has been identified, whose function remains unknown. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008).
  • Alternative names
    • HCV antibody
    • HCV Non-structural protein 5A antibody
    • Non-structural protein 5A antibody
    • NS5A antibody
    • p56 antibody
    see all

Anti-Hepatitis C Virus NS5a antibody [388] images

  • All lanes : Anti-Hepatitis C Virus NS5a antibody [388] (ab20342) at 1/1000 dilution

    Lane 1 : Huh-7 (negative control)
    Lane 2 : HCV Replicon cell line

    Secondary
    Goat anti-mouse IgG conjugated to HRP

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Hepatitis C Virus NS5a antibody [388] (ab20342)

This product has been referenced in:
  • El Abd YS  et al. Neutralizing activities of caprine antibodies towards conserved regions of the HCV envelope glycoprotein E2. Virol J 8:391 (2011). WB ; Hepatitis C Virus . Read more (PubMed: 21819575) »
  • Gonzalez O  et al. The heat shock protein inhibitor Quercetin attenuates hepatitis C virus production. Hepatology 50:1756-64 (2009). IP . Read more (PubMed: 19839005) »

See all 2 Publications for this product

Product Wall

Thanks for sending along your protocol.

1. ab49033 is unlikely to be detected by these antibodies as it only contains about 100 aa of the total protein. As described on datasheet this product containsamino acids"corresponding to amino ac...

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Thank you for contacting us.

This may sound familiar ;-)

This anti-HCVNS4b and NS5aantibodies are mouse monoclonals of IgG2b andIgG2a isotype, respectively.IgG2 antibodies usually comprise 450 amino acids, resulting in a molecular w...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (huh-7 cells replicon)
Specification huh-7 cells replicon
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Jul 30 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (liver cell line)
Loading amount 50000 cells
Specification liver cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

Submitted Jun 28 2006

Thank you for your enquiry. The molecular weight of native HCV NS-5a is a 56kDa. I hope this information helps. Please do not hesitate to contacts us if you need anything further.

Thank you for your enquiry. Ab20342 has not been tested for reactivity to genotype 2a. Also, the epitope has not been determined. Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"