Anti-Hepatitis C Virus NS5a antibody [H26] (ab13833)

Overview

  • Product name
    Anti-Hepatitis C Virus NS5a antibody [H26]
  • Description
    Mouse monoclonal [H26] to Hepatitis C Virus NS5a
  • Host species
    Mouse
  • Specificity
    Hepatitis C Virus antigen NS5a. This antibody works with the HCV genotypes 1a, 1b, 3 and 4, but not with genotype 2a.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Immunogen

    Recombinant full length protein (E. coli).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    H26
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/1000.
ICC/IF 1/50.

Target

  • Relevance
    HCV is classified into the genus Hepacivirus of the family Flaviviridae. Like all the members of the family, HCV is an enveloped, single-stranded, positive-sense RNA virus. Its genome (about 9600 nt) is flanked at both termini by conserved, highly structured non-translated regions (NTRs) and encodes a polyprotein precursor (about 3000 aa), which is proteolytically processed by host and viral proteases to produce the structural (core, E1, E2 and p7) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B) proteins of the virus. Recently, an additional protein has been identified, whose function remains unknown. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008).
  • Alternative names
    • HCV antibody
    • HCV Non-structural protein 5A antibody
    • Non-structural protein 5A antibody
    • NS5A antibody
    • p56 antibody
    see all

Images

  • Representative western blot detecting Hepatitis C Virus NS5a with ab13833.

    Amounts of Hepatitis C Virus NS5a were compared to Core after spread of infection to >80% of Huh7.5 cells transfected with H77C(1a) and P346A/P351A/P354A, delta414-428 or “delta414-428+20aa” mutants.

References

This product has been referenced in:
  • Madhvi A  et al. A screen for novel hepatitis C virus RdRp inhibitor identifies a broad-spectrum antiviral compound. Sci Rep 7:5816 (2017). Read more (PubMed: 28725041) »
  • Abdallah C  et al. Hepatitis C virus core protein targets 4E-BP1 expression and phosphorylation and potentiates Myc-induced liver carcinogenesis in transgenic mice. Oncotarget 8:56228-56242 (2017). Read more (PubMed: 28915586) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for your message.

Reviewing the recommended storage details for these antibodies, I can suggest they should not be significantly affected by short term storage for a week or two at +4oC. However, I would recommend to aliquot and fr...

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Thank you for your enquiry. I can confirm that the exact epitope is not know, but it has been mapped between amino acid positions 2200 and 2250. I hope this will be helpful to you. If you have any further questions, please do not hesitate to cont...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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