Anti-Hepatocyte Specific Antigen antibody [OCH1E5] (ab190706)
Key features and details
- Mouse monoclonal [OCH1E5] to Hepatocyte Specific Antigen
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Hepatocyte Specific Antigen antibody [OCH1E5]
See all Hepatocyte Specific Antigen primary antibodies -
Description
Mouse monoclonal [OCH1E5] to Hepatocyte Specific Antigen -
Host species
Mouse -
Specificity
Hepatocyte specific antigens are useful in studying hepatocellular tumors. They may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. They have been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. Hepatcoyte labels an antigen in the mitochondrial fraction of the liver homogenates. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Hepatocyte Specific Antigen. Extract of a formalin-fixed, rejected-allograft of a Human liver.
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Positive control
- IHC: Liver or Hepatocellular Carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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Purification notes
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G -
Clonality
Monoclonal -
Clone number
OCH1E5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. |
Target
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Relevance
Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hepatocellular Carcinoma tissue labelling Hepatocyte Specific Antigen with ab190706 at 1-2 µg/mL for 30 minutes at room temperature. Tissues were heated in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Liver tissue labelling Hepatocyte Specific Antigen with ab190706 at 1-2 µg/mL for 30 minutes at room temperature. Tissues were heated in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab190706 has been referenced in 4 publications.
- Song G et al. Single-cell transcriptomic analysis suggests two molecularly subtypes of intrahepatic cholangiocarcinoma. Nat Commun 13:1642 (2022). PubMed: 35347134
- Bertol BC et al. HLA-G liver expression and HLA-G extended haplotypes are associated with chronic hepatitis C in HIV-negative and HIV-coinfected patients. Clin Immunol 217:108482 (2020). PubMed: 32470543
- Zhang J et al. Overexpression of Epcam and CD133 Correlates with Poor Prognosis in Dual-phenotype Hepatocellular Carcinoma. J Cancer 11:3400-3406 (2020). PubMed: 32231746
- Xue R et al. Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes. Cancer Cell 35:932-947.e8 (2019). PubMed: 31130341