Anti-Hepatocyte Specific Antigen antibody [OCH1E5], prediluted (ab75677)

Overview

  • Product name
    Anti-Hepatocyte Specific Antigen antibody [OCH1E5], prediluted
    See all Hepatocyte Specific Antigen primary antibodies
  • Description
    Mouse monoclonal [OCH1E5] to Hepatocyte Specific Antigen, prediluted
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Formalin fixed failed human allograft liver that was mechanically disrupted

  • Positive control
    • Human Liver and Liver carcinoma.

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Tris HCl, pH 7.6 containing stabilising protein
  • Concentration information loading...
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    OCH1E5
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Target

  • Relevance
    Hepatocyte specific antigen is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. Hepatcoyte labels an antigen in the mitochondrial fraction of the liver homogenates. Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
  • Cellular localization
    Cytoplasmic

Images

  • Formalin-fixed, paraffin-embedded human liver stained with ab75677 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of hepatocytes.

References

This product has been referenced in:
  • Liu W  et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett 11:3669-3675 (2016). IHC ; Human . Read more (PubMed: 27313680) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us.
We do not have this information available yet, customer need to search literature for finding suitable protein target.
We however have antibodies against hepatocellular markers that are mouse specific e.g. ab46819...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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