Our Abpromise guarantee covers the use of ab75883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 3 kDa).Can be blocked with Human Hepcidin-25 peptide (ab31875). No data on endogenous protein, only tested in recombinant protein.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|