Recombinant
RabMAb

Anti-Hepcidin-25 antibody [EPR18074] (ab187778)

Overview

  • Product name
    Anti-Hepcidin-25 antibody [EPR18074]
    See all Hepcidin-25 primary antibodies
  • Description
    Rabbit monoclonal [EPR18074] to Hepcidin-25
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    within Human Hepcidin-25 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P81172

  • Positive control
    • WB: Human Hepcidin fragment protein; Human plasma, serum and liver lysate; Rat serum treated with LPS.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab187778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 9, 3 kDa (predicted molecular weight: 9 kDa).

Target

  • Function
    Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
    Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
  • Tissue specificity
    Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
  • Involvement in disease
    Hemochromatosis 2B
  • Sequence similarities
    Belongs to the hepcidin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Form
    Hepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure
  • Alternative names
    • Hamp antibody
    • HEPC antibody
    • HEPC_HUMAN antibody
    • Hepc20 antibody
    • Hepc25 antibody
    • HEPCIDIN antibody
    • Hepcidin 20 antibody
    • Hepcidin 25 antibody
    • Hepcidin antimicrobial peptide antibody
    • Hepcidin-20 antibody
    • Hepcidin25 antibody
    • HFE2 antibody
    • HFE2B antibody
    • LEAP 1 antibody
    • LEAP-1 antibody
    • LEAP1 antibody
    • Liver expressed antimicrobial peptide antibody
    • Liver-expressed antimicrobial peptide 1 antibody
    • PLTR antibody
    • Putative liver tumor regressor antibody
    see all

Images

  • Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/5000 dilution + Human Hepcidin fragment protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 9 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 second

    Blocking/Dilution buffer: 5% NFDM/TBST.

    In house recombinant Human Hepcidin is a His-tagged fragment protein (aa25-84).

  • All lanes : Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human serum
    Lane 3 : Human liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution

    Predicted band size : 9 kDa
    Observed band size : 3 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 1% NFDM/TBST.

  • Lanes 1 - 2 : Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution
    Lanes 3 - 4 : Anti-Hepcidin-25 antibody (ab30760) at 1/1000 dilution

    Lane 1 : Rat serum treated with LPS at 30 µg
    Lane 2 : Rat serum treated with LPS at 10 µg
    Lane 3 : Rat serum treated with LPS at 30 µg
    Lane 4 : Rat serum treated with LPS at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution

    Predicted band size : 9 kDa
    Observed band size : 3,10 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 1% NFDM/TBST.

    ab187778 has similar reactivity to rabbit polyclonal ab30760.

References

ab187778 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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