HepG2 whole cell lysate (IL6 stimulated) (ab14833)

Overview

  • Product nameHepG2 whole cell lysate (IL6 stimulated)
    See all HepG2 lysates
  • Description
    HepG2 whole cell lysate
  • General notesCell line: HepG2 (Human hepatocellular carcinoma).
    We recommend aliquoting the extracts into single use fractions and then storing them.
  • Tested applicationsSuitable for: WB, EMSAmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.90
    Constituents: 0.48% HEPES, 0.75% Potassium chloride, 0.01% Magnesium chloride, 20% Glycerol, 0.009% PMSF, 0.008% DTT
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundHepatitis B virus (HBV) is the major cause of acute and chronic hepatitis, leading to progressive development of necroinflammatory changes in the liver, which can result in cirrhosis and hepatocellular carcinoma. Although the development of an effective vaccine to prevent HBV infection has shown promising results and should lead to its eventual eradication, antiviral chemotherapy remains the only effective method to prevent the progression of the disease in chronic carriers. Therefore, the development of new antiretroviral agents active against HBV is needed. HepG2 cells have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies (in particular signalling pathway studies).

Applications

Our Abpromise guarantee covers the use of ab14833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
EMSA Use at an assay dependent dilution.

References for HepG2 whole cell lysate (IL6 stimulated) (ab14833)

ab14833 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. We would normally recommend using 20-40 micrograms (ug) of protein per lane, therefore, I would suggest starting with 20ug. If the band you obtain is too weak, you can increase the protein amount, or if the band is too s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"