The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).
May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.
Detected in breast, colon, bone trabecular cells and fibroblasts.
Belongs to the multicopper oxidase family. Contains 6 plastocyanin-like domains.
Western blot analysis of rat tissue lysate (20μg/lane) labelling Hephaestin with ab95137 at 1/1000 in 0.1xTBST plus milk for 24 hours at 4ºC. A HRP conjugated Anti-rabbit polyclonal (1/3000) was used as the secondary antibody.