Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab95137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).

Target

  • Function
    May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.
  • Tissue specificity
    Detected in breast, colon, bone trabecular cells and fibroblasts.
  • Sequence similarities
    Belongs to the multicopper oxidase family.
    Contains 6 plastocyanin-like domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPL antibody
    • Heph antibody
    • HEPH_HUMAN antibody
    • Hephaestin antibody

Images

  • Anti-Hephaestin antibody (ab95137) at 1 µg/ml + WI-38 whole cell lysate (ab3960) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 130 kDa
    Observed band size : 130 kDa


    Exposure time : 20 minutes
  • All lanes : Anti-Hephaestin antibody (ab95137) at 1/1000 dilution

    Lane 1 : adult rat cortex
    Lane 2 : neo-natal rat heart
    Lane 3 : 3 month old rat heart
    Lane 4 : rat embryo

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated Anti-rabbit polyclonal at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 130 kDa
    Observed band size : 130 kDa
    Additional bands at : 17 kDa (possible non-specific binding),64 kDa (possible non-specific binding).

    Western blot analysis of rat tissue lysate (20μg/lane) labelling Hephaestin with ab95137 at 1/1000 in 0.1xTBST plus milk for 24 hours at 4ºC. A HRP conjugated Anti-rabbit polyclonal (1/3000) was used as the secondary antibody.

    See Abreview

References

ab95137 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (Duodenum)
Specification
Duodenum
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain, heart, embryo)
Loading amount
20 µg
Specification
brain, heart, embryo
Gel Running Conditions
Reduced Denaturing (4-12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Oct 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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