Anti-Hepsin antibody (ab31148)


  • Product nameAnti-Hepsin antibody
    See all Hepsin primary antibodies
  • Description
    Rabbit polyclonal to Hepsin
  • SpecificityThis antibody specifically recognized recombinant human hepsin in Western blot analysis
  • Tested applicationsSuitable for: IHC-P, ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human).



Our Abpromise guarantee covers the use of ab31148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/50000.
WB Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionPlays an essential role in cell growth and maintenance of cell morphology.
  • Tissue specificityPresent in most tissues, with the highest level in liver.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 1 SRCR domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.4.21. antibody
    • HEPS antibody
    • HEPS_HUMAN antibody
    • Hepsin antibody
    • Hpn antibody
    • Serine protease hepsin antibody
    • Serine protease hepsin catalytic chain antibody
    • Serine protease hepsin non catalytic chain antibody
    • TMPRSS 1 antibody
    • TMPRSS1 antibody
    • Transmembrane protease serine 1 antibody
    see all

Anti-Hepsin antibody images

  • IHC image of ab31148 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31148, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Hepsin antibody (ab31148)

ab31148 has not yet been referenced specifically in any publications.

Product Wall

Our supplier for this antibody does not have a protocol for flow cytometry from the laboratory that produced it, though they were assured that it had been tested and shown to work for this application. I am disappointed that I am not able to provide th...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (prostate cancer cell lines, transfected 293 cell l)
Loading amount 20 µg
Specification prostate cancer cell lines, transfected 293 cell l
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 05 2010