The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml. IHC-P: Use at a concentration of 3 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
RelevanceHERC3 (Hect domain and RLD 3) belongs to the HERC family of ubiquitin ligases which contain a HECT domain and at least 1 RCC1-like domain (RLD). E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. The RLD domain is predicted to act as a guanine nucleotide exchange factor for small G proteins.
Cellular localizationCytoplasmic. Cytoplasmic vesicle. Note: Also found in vesicular-like structures.