Overview

  • Product nameAnti-HERC6 antibody
    See all HERC6 primary antibodies
  • Description
    Rabbit polyclonal to HERC6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 123 - 393 of Human HERC6 (BC035775).

  • Positive control
    • WB: HeLa cell lysate. IHC-P: fetal testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab99949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IHC-P 1/100 - 1/500.

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
  • Tissue specificityDetected in brain, heart, placenta and testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 5 RCC1 repeats.
  • Developmental stageExpressed in fetal brain.
  • Cellular localizationCytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 6.3.2. antibody
    • FLJ20637 antibody
    • HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 antibody
    • HECT domain and RCC1 like domain containing protein 6 antibody
    • HECT domain and RCC1-like domain 6 antibody
    • HECT domain and RCC1-like domain-containing protein 6 antibody
    • Hect domain and RLD 6 antibody
    • HERC 6 antibody
    • HERC6 antibody
    • HERC6_HUMAN antibody
    • Potential ubiquitin ligase antibody
    • Probable E3 ubiquitin protein ligase HERC6 antibody
    • Probable E3 ubiquitin-protein ligase HERC6 antibody
    see all

Anti-HERC6 antibody images

  • Anti-HERC6 antibody (ab99949) at 1/1000 dilution + HeLa cell lysate

    Predicted band size : 115 kDa
    Observed band size : 115 kDa
  • ab99949 at 1/100 dilution detecting HERC6 expression in formalin-fixed, paraffin-embedded Human fetal testis tissue.
    Note cytoplasmic staining.

References for Anti-HERC6 antibody (ab99949)

ab99949 has not yet been referenced specifically in any publications.

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Thank you for your enquiry.
I am sorry to hear this vial of ab99949 is giving you trouble. I am curious to know if you have successfully blotted the HeLa cell lysate with any other antibodies.
Thanks in advance for the additional information!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"