Overview

  • Product nameAnti-HERPUD1 antibody
    See all HERPUD1 primary antibodies
  • Description
    Rabbit polyclonal to HERPUD1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HERPUD1.

    (Peptide available as ab73668.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Colon, Rat Colon, Human Colon, Mouse Skeletal Muscle, Rat Skeletal Muscle, Human Skeletal Muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab73669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionComponent of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Could enhance presenilin-mediated beta-amyloid protein 40 generation.
  • Tissue specificityWidely expressed; in the brain, expression seems to be restricted to neurons and vascular smooth muscle cells. Present in activated microglia in senile plaques in the brain of patients with Alzheimer disease.
  • Sequence similaritiesContains 1 ubiquitin-like domain.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HERP antibody
    • HERP1_HUMAN antibody
    • HERPUD1 antibody
    • Homocysteine inducible endoplasmic reticulum stress inducible ubiquitin like domain member 1 antibody
    • Homocysteine inducible endoplasmic reticulum stress inducible ubiquitin like domain member 1 protein antibody
    • Homocysteine responsive endoplasmic reticulum resident ubiquitin like domain member 1 antibody
    • Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein antibody
    • KIAA0025 antibody
    • Methyl methanesulfonate (MMF) inducible fragment protein 1 antibody
    • Methyl methanesulfonate (MMF)-inducible fragment protein 1 antibody
    • MIF1 antibody
    • MMS inducible antibody
    • SUP antibody
    see all

Anti-HERPUD1 antibody images

  • All lanes : Anti-HERPUD1 antibody (ab73669) at 1 µg/ml

    Lane 1 : Colon (Mouse) Tissue Lysate
    Lane 2 : Colon (Rat) Tissue Lysate
    Lane 3 : Human colon tissue lysate - total protein (ab30051)
    Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 5 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 85 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab73669 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73669, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

References for Anti-HERPUD1 antibody (ab73669)

This product has been referenced in:
  • Zhang R  et al. Zhangfei/CREB-ZF - A Potential Regulator of the Unfolded Protein Response. PLoS One 8:e77256 (2013). ICC/IF ; Human . Read more (PubMed: 24155933) »

See 1 Publication for this product

Product Wall

Thank you for taking the time to contact us. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that ab73669 is tested and covered by our 6 month guarantee for u...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (macrophages (P388D1))
Specification macrophages (P388D1)
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (epithelial colorectal adenocarcinoma (Caco))
Specification epithelial colorectal adenocarcinoma (Caco)
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"