Overview

  • Product nameAnti-Hes1 antibody [2D2]
    See all Hes1 primary antibodies
  • Description
    Mouse monoclonal [2D2] to Hes1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human Hes1 produced in HEK293T cells (NP_005515).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA; COS7 cells transiently transfected by pCMV6-ENTRY Hes1 cDNA; Jurkat cells; Human thyroid, thyroid carcinoma and bladder carcinoma tissues.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab119776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 30 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
    The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb39 antibody
    • C-HAIRY1 antibody
    • c-hairy1A antibody
    • Class B basic helix-loop-helix protein 39 antibody
    • FLJ20408 antibody
    • Hairy and enhancer of split 1 (Drosophila) antibody
    • Hairy and enhancer of split 1 antibody
    • Hairy homolog (Drosophila) antibody
    • Hairy homolog antibody
    • Hairy like antibody
    • Hairy, Drosophila, homolog of antibody
    • Hairy-like protein antibody
    • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
    • HAIRY1 antibody
    • HES-1 antibody
    • hes1 antibody
    • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
    • HES1_HUMAN antibody
    • HHL antibody
    • HL antibody
    • HRY antibody
    • MGC129109 antibody
    • OTTHUMP00000209031 antibody
    • RHL antibody
    • Transcription factor HES-1 antibody
    see all

Anti-Hes1 antibody [2D2] images

  • All lanes : Anti-Hes1 antibody [2D2] (ab119776) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 30 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211709)
  • ab119776, at 1/100 dilution, staining Hes1 in COS7 cells transiently transfected with pCMV6-ENTRY Hes1 cDNA by Immunofluorescence.
  • ab119776, at 1/100 dilution, staining Hes1 (red) in Jurkat cells, compared to a nonspecific negative control antibody,(blue) by Flow cytometry.
  • ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
  • ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human thyroid tissue by Immunohistochemistry.

References for Anti-Hes1 antibody [2D2] (ab119776)

ab119776 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Peritoneal tumor)
Permeabilization Yes - 0.3% Triton X 100
Specification Peritoneal tumor
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 25 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Human Cell (Colon)
Specification Colon
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"