Anti-Hes1 antibody (ab55265)




Our Abpromise guarantee covers the use of ab55265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
    The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb39 antibody
    • C-HAIRY1 antibody
    • c-hairy1A antibody
    • Class B basic helix-loop-helix protein 39 antibody
    • FLJ20408 antibody
    • Hairy and enhancer of split 1 (Drosophila) antibody
    • Hairy and enhancer of split 1 antibody
    • Hairy homolog (Drosophila) antibody
    • Hairy homolog antibody
    • Hairy like antibody
    • Hairy, Drosophila, homolog of antibody
    • Hairy-like protein antibody
    • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
    • HAIRY1 antibody
    • HES-1 antibody
    • hes1 antibody
    • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
    • HES1_HUMAN antibody
    • HHL antibody
    • HL antibody
    • HRY antibody
    • MGC129109 antibody
    • OTTHUMP00000209031 antibody
    • RHL antibody
    • Transcription factor HES-1 antibody
    see all

Anti-Hes1 antibody images

  • Predicted band size : 30 kDa
  • Overlay histogram showing HepG2 cells stained with ab55265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55265, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Hes1 antibody (ab55265)

ab55265 has not yet been referenced specifically in any publications.

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I have issued a free of charge replacement for one vialof ab108937.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is...

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Unfortunately neither of these numers correspond to an order from Sapphire for a vial of ab55265. Please check the Abcam order referenc number or your internal PO number. Also it would be helpful to know when the order ...

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I recommend switching to the rabbit monoclonal, ab108937.

Click here (or use the following:

For blocking we recommend 5% milk in Tris-buffered saline with 0.1% Triton X-100 or Twe...

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Thanks for your reply.

I'm happy to offer a replacement for this vial of ab55265 as it does seem to not be working as expected. Since you have purchased the product through Sapphire, please contact them regarding this issue, letting them know...

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My colleague is out of the office today but will be back next week to follow up with you. Your protocol and samples seem appopriate to me. I expect Jeremy will want to know if you have been able to obtain good western results for these samples with oth...

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Thank you for your enquiry. I am sorry to hear of the trouble you are having with your western blot.Tohelp you most efficiently, please reply with answers to the followiing questions regarding your western blot experiment. Thanks!!

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Thank you for your enquiry. I am sorry to confirm that Hes1 is a transcription factor that is fairly ubiquitously expressed.  It is therefore difficult to suggest a negative control. Unfortunately, this antibody has been tested externally and a neg...

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Application Western blot
Sample Human Cell lysate - whole cell (LNCaP Cell line)
Loading amount 40 µg
Specification LNCaP Cell line
Treatment Ethanol and 100 nM DHT
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 23 2009

Thank you for your recent telephone enquiry. From a literature search, I can see that the Hes1 protein can be part of the notch signalling pathway. This particular publication states that NOTCH receptors can signal through the hairy and enhancer of ...

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