- Datasheet
- Specific References (19)
- Protocols
Overview
- Product nameAnti-Hes1 antibody
See all Hes1 primary antibodies - DescriptionRabbit polyclonal to Hes1
- SpecificityThis antibody reacts specifically with mouse 30 kDa Hes1 protein.
- Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Immunogen
Synthetic peptide corresponding to Human Hes1 (C terminal).
Database link: Q14469
(Peptide available asab126460)
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
- Storage bufferConstituent: Water
- PurityWhole antiserum
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab71559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/200 - 1/2000. Predicted molecular weight: 30 kDa.Can be blocked with Hes1 peptide (ab126460). |
Target
- FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
- Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. - DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity. - Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 3280 Human
- Entrez Gene: 15205 Mouse
- Entrez Gene: 29577 Rat
- Omim: 139605 Human
- SwissProt: Q14469 Human
- SwissProt: P35428 Mouse
- SwissProt: Q04666 Rat
- Unigene: 250666 Human
see all -
Alternative names
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
Anti-Hes1 antibody images
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Western blot - Anti-Hes1 antibody (ab71559)This image is courtesy of an anonymous AbreviewAnti-Hes1 antibody (ab71559) at 1/2000 dilution + MCF-7 at 20 µg
Secondary
HRP conjugated goat anti-rabbit at 1/20000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 30 kDa
Exposure time : 15 secondsThis image is courtesy of an anonymous Abreview
Blocked with 5% milk at RT
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Immunohistochemistry (Frozen sections) - Anti-Hes1 antibody (ab71559)This image is courtesy of an anonymous Abreviewab71559 staining Hes1 in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone/methanol (1:1), permeabilized with PBS + triton X100 (0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody (ab71559)This image is courtesy of an anonymous Abreviewab71559 staining Hes1 in human pancreatic cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye and subjected to heat-mediated antigen retrieval in citric buffer prior to permeabilising in PBS + Triton X-100 0.025% and blocking with 10% serum for 1 hour at RT. The primary antibody was diluted 1/250 and incubated with the sample for 8 hours at 4°C. An biotin-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000. -
Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody (ab71559)This image is courtesy of an anonymous Abreviewab71559 staining Hes1 in the mouse NIH3T3 cell line from by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 10% serum for 30 minutes. Samples were incubated with primary antibody (1/200) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
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Western blot - Anti-Hes1 antibody (ab71559)All lanes : Anti-Hes1 antibody (ab71559)
Lane 1 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 2 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 3 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 4 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 5 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 6 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 7 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Lane 8 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Predicted band size : 30 kDa
Actin blot given as control. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody (ab71559)This image is courtesy of an Anonymous Abreview.ab71559 staining Hes1 in small intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200.
References for Anti-Hes1 antibody (ab71559)
This product has been referenced in:
- Tang J et al. The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer. J Exp Clin Cancer Res 35:91 (2016). WB ; Human . Read more (PubMed: 27287498) »
- Nishio M et al. Dysregulated YAP1/TAZ and TGF-ß signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci U S A 113:E71-80 (2016). WB ; Mouse . Read more (PubMed: 26699479) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"