Overview

  • Product name
  • Description
    Rabbit polyclonal to Hes1
  • Host species
    Rabbit
  • Specificity
    This antibody reacts specifically with mouse 30 kDa Hes1 protein.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Hes1 (C terminal).
    Database link: Q14469
    (Peptide available as ab126460)

Properties

Applications

Our Abpromise guarantee covers the use of ab71559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 30 kDa.Can be blocked with Hes1 peptide (ab126460).

Target

  • Function
    Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Domain
    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
    The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb39 antibody
    • C-HAIRY1 antibody
    • c-hairy1A antibody
    • Class B basic helix-loop-helix protein 39 antibody
    • FLJ20408 antibody
    • Hairy and enhancer of split 1 (Drosophila) antibody
    • Hairy and enhancer of split 1 antibody
    • Hairy homolog (Drosophila) antibody
    • Hairy homolog antibody
    • Hairy like antibody
    • Hairy, Drosophila, homolog of antibody
    • Hairy-like protein antibody
    • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
    • HAIRY1 antibody
    • HES-1 antibody
    • hes1 antibody
    • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
    • HES1_HUMAN antibody
    • HHL antibody
    • HL antibody
    • HRY antibody
    • MGC129109 antibody
    • OTTHUMP00000209031 antibody
    • RHL antibody
    • Transcription factor HES-1 antibody
    see all

Images

  • Anti-Hes1 antibody (ab71559) at 1/2000 dilution + MCF-7 at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 30 kDa


    Exposure time: 15 seconds


    Blocked with 5% milk at RT

    See Abreview

  • ab71559 staining Hes1 in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone/methanol (1:1), permeabilized with PBS + triton X100 (0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab71559 staining Hes1 in human pancreatic cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye and subjected to heat-mediated antigen retrieval in citric buffer prior to permeabilising in PBS + Triton X-100 0.025% and blocking with 10% serum for 1 hour at RT. The primary antibody was diluted 1/250 and incubated with the sample for 8 hours at 4°C. An biotin-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000.

    See Abreview

  • ab71559 staining Hes1 in the mouse NIH3T3 cell line from by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 10% serum for 30 minutes. Samples were incubated with primary antibody (1/200) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Hes1 antibody (ab71559)

    Lane 1 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
    Lane 2 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
    Lane 3 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
    Lane 4 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
    Lane 5 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
    Lane 6 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
    Lane 7 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
    Lane 8 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)

    Predicted band size: 30 kDa



    Actin blot given as control.
  • ab71559 staining Hes1 in small intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

References

This product has been referenced in:
  • Hu S  et al. The function of Notch1 intracellular domain in the differentiation of gastric cancer. Oncol Lett 15:6171-6178 (2018). Read more (PubMed: 29616098) »
  • Ding Y  et al. Deubiquitinase inhibitor b-AP15 activates endoplasmic reticulum (ER) stress and inhibits Wnt/Notch1 signaling pathway leading to the reduction of cell survival in hepatocellular carcinoma cells. Eur J Pharmacol 825:10-18 (2018). Read more (PubMed: 29454609) »

See all 40 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T, K562, MCF7, HeLa)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
15 µg
Specification
293T, K562, MCF7, HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 02 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231)
Permeabilization
Yes - tween-20
Specification
MDA-MB-231
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Jan 02 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (paraffin-embedded human colon carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Specification
paraffin-embedded human colon carcinoma
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 02 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 31 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Oct 19 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted Aug 12 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Mar 22 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS1 cell line)
Specification
COS1 cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2013

Thank you for contacting us. I am sorry you are experiencing difficulty with this product. From the Abreview itself, there is a link next to "anaonymous" that says "click here to contact reviewer."

I appreciate the time taken to submit further information to us.

Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results.

I apologise for the inconvenience and am pleased t...

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