Anti-Hes1 antibody - Aminoterminal end (ab70933)


  • Product nameAnti-Hes1 antibody - Aminoterminal end
    See all Hes1 primary antibodies
  • Description
    Rabbit polyclonal to Hes1 - Aminoterminal end
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the N-terminal region of human Hes1.

  • Positive control
    • HL60 cell line lysates.



Our Abpromise guarantee covers the use of ab70933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/1000.
    WB: 1/50 - 1/100. Detects a band of approximately 29 kDa plus a range of other bands. (predicted molecular weight: 29 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 Orange domain.
    • DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
      The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
      The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHb39 antibody
      • C-HAIRY1 antibody
      • c-hairy1A antibody
      • Class B basic helix-loop-helix protein 39 antibody
      • FLJ20408 antibody
      • Hairy and enhancer of split 1 (Drosophila) antibody
      • Hairy and enhancer of split 1 antibody
      • Hairy homolog (Drosophila) antibody
      • Hairy homolog antibody
      • Hairy like antibody
      • Hairy, Drosophila, homolog of antibody
      • Hairy-like protein antibody
      • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
      • HAIRY1 antibody
      • HES-1 antibody
      • hes1 antibody
      • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
      • HES1_HUMAN antibody
      • HHL antibody
      • HL antibody
      • HRY antibody
      • MGC129109 antibody
      • OTTHUMP00000209031 antibody
      • RHL antibody
      • Transcription factor HES-1 antibody
      see all

    Anti-Hes1 antibody - Aminoterminal end images

    • Anti-Hes1 antibody - Aminoterminal end (ab70933) at 1/60 dilution + HL60 cell line lysates at 35 µg

      Predicted band size : 29 kDa
      Observed band size : 29 kDa

    References for Anti-Hes1 antibody - Aminoterminal end (ab70933)

    ab70933 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (pancreatic cancer cells (from cell lines))
    Loading amount 75 µg
    Specification pancreatic cancer cells (from cell lines)
    Treatment DAPT (gamma-secretase inhibitor)
    Gel Running Conditions Reduced Denaturing (12.5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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    Submitted Jul 07 2010