Overview

  • Product name
    Anti-HES4 antibody
  • Description
    Rabbit polyclonal to HES4
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: AADTPGKPSA SPMAGAPASA SRTPDKPRSA AEHRKSSKPV MEKRRRARIN , corresponding to amino acids 2-51 of Human HES4

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Relevance
    HES4 may be a transcriptional repressor. It is expressed in stomach, pancreas, ovary, uterus, eye and bone. It has an N-terminal bHLH domain.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • bHLH factor Hes4 antibody
    • Hairy and enhancer of split 4 antibody
    • Transcription factor HES 4 antibody

Images

  • Anti-HES4 antibody (ab66377) at 2 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 24 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

References

ab66377 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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