Overview

  • Product name
    Anti-HES5 antibody
    See all HES5 primary antibodies
  • Description
    Rabbit polyclonal to HES5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human HES5 (Q5TA89)

  • Positive control
    • Mouse liver tissue lysate and Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab107593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 6 - 9 µg/ml. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Transcriptional repressor of genes that require a bHLH protein for their transcription.
  • Tissue specificity
    Expressed in fetal heart and brain tumors.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Domain
    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • bHLHb38 antibody
    • Class B basic helix-loop-helix protein 38 antibody
    • Hairy and enhancer of split 5 antibody
    • Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
    • HES 5 antibody
    • hes family bHLH transcription factor 5 antibody
    • Hes5 antibody
    • HES5 protein antibody
    • HES5_HUMAN antibody
    • MGI:104876 antibody
    • Transcription factor HES-5 antibody
    see all

Images

  • Western blot - Anti-HES5 antibody (ab107593)
    Western blot - Anti-HES5 antibody (ab107593)
    All lanes : Anti-HES5 antibody (ab107593) at 7 µg/ml

    Lane 1 : HES5 in mouse liver tissue
    Lane 2 : HES5 in mouse liver tissue with immunizing peptide

    Secondary
    All lanes : Goat anti-rabbit Ig HRP

    Developed using the ECL technique.

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody (ab107593)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody (ab107593)
    Immunohistochemical analysis of HES5 in formalin-fixed, paraffin-embedded human brain tissue using ab107593 at 5 µg/ml.

References

This product has been referenced in:
  • Chen Y  et al. Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination. PLoS One 12:e0189380 (2017). Read more (PubMed: 29253893) »

See 1 Publication for this product

Publishing research using ab107593? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

Muchas gracias por la oferta que me has hecho.
Solo estoy pendiente de hacer la inmunohistoquímica de esos tres anticuerpos para entregar y dar por finalizado un trabajo de muchas muestras, muchos otros anticuerpos y la biología mole...

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Gracias por tu respuesta.

Todos nuestros anticuerpos y demás productos están garantizados y deben funcionar en las aplicaciones y especies indicadas en la datasheet de los mismos. Por eso todos tienen una garantía de 6 me...

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Muchas gracias por tu contestación,
No he mencionado el tiempo de fijación, puesto que los bloques, nos los proporciona el servicio de anatomía patológica, quefueron utilizados primeropara diagnostico y doy porsupuesto ...

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Muchas gracias por tu respuesta y facilitarnos detalles adicionales.

Siento mucho que los anticuerpos no hayan funcionado y las molestias ocasionadas. Después de estudiar el protocolo y atendiendo a las imágenes, estoy dispuesta...

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