Customer reviews and FAQs

Overview

  • Product name
    Anti-HES5 antibody
    See all HES5 primary antibodies
  • Description
    Rabbit polyclonal to HES5
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human HES5 (Q5TA89)

  • Positive control
    • Mouse liver tissue lysate and Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab107593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 6 - 9 µg/ml. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Transcriptional repressor of genes that require a bHLH protein for their transcription.
  • Tissue specificity
    Expressed in fetal heart and brain tumors.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Domain
    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • bHLHb38 antibody
    • Class B basic helix-loop-helix protein 38 antibody
    • Hairy and enhancer of split 5 antibody
    • Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
    • HES 5 antibody
    • hes family bHLH transcription factor 5 antibody
    • Hes5 antibody
    • HES5 protein antibody
    • HES5_HUMAN antibody
    • MGI:104876 antibody
    • Transcription factor HES-5 antibody
    see all

Anti-HES5 antibody images

  • Western blot - HES5 antibody (ab107593)
    Western blot - HES5 antibody (ab107593)
    All lanes : Anti-HES5 antibody (ab107593) at 7 µg/ml

    Lane 1 : HES5 in mouse liver tissue
    Lane 2 : HES5 in mouse liver tissue with immunizing peptide

    Secondary
    Goat anti-rabbit Ig HRP
    Developed using the ECL technique

    Predicted band size : 18 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HES5 antibody (ab107593)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HES5 antibody (ab107593)
    Immunohistochemical analysis of HES5 in formalin-fixed, paraffin-embedded human brain tissue using ab107593 at 5 µg/ml.

References for Anti-HES5 antibody (ab107593)

ab107593 has not yet been referenced specifically in any publications.

Publishing research using ab107593? Please let us know so that we can cite the reference in this datasheet.

Product Wall

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2 Abreviews or Q&A

10
Votes: Highest First

Muchas gracias por la oferta que me has hecho.
Solo estoy pendiente de hacer la inmunohistoquímica de esos tres anticuerpos para entregar y dar por finalizado un trabajo de muchas muestras, muchos otros anticuerpos y la biología mole...

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Gracias por tu respuesta.

Todos nuestros anticuerpos y demás productos están garantizados y deben funcionar en las aplicaciones y especies indicadas en la datasheet de los mismos. Por eso todos tienen una garantía de 6 me...

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Muchas gracias por tu contestación,
No he mencionado el tiempo de fijación, puesto que los bloques, nos los proporciona el servicio de anatomía patológica, quefueron utilizados primeropara diagnostico y doy porsupuesto ...

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Muchas gracias por tu respuesta y facilitarnos detalles adicionales.

Siento mucho que los anticuerpos no hayan funcionado y las molestias ocasionadas. Después de estudiar el protocolo y atendiendo a las imágenes, estoy dispuesta...

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