Our Abpromise guarantee covers the use of ab25374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/200 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-Fr 1/200 - 1/1000.

This product has not been tested with paraffin sections.


  • FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription.
  • Tissue specificityExpressed in fetal heart and brain tumors.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb38 antibody
    • Class B basic helix-loop-helix protein 38 antibody
    • Hairy and enhancer of split 5 antibody
    • Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
    • HES 5 antibody
    • hes family bHLH transcription factor 5 antibody
    • Hes5 antibody
    • HES5 protein antibody
    • HES5_HUMAN antibody
    • MGI:104876 antibody
    • Transcription factor HES-5 antibody
    see all

Anti-HES5 antibody images

  • ab25374 staining the cortex of e14.5 mouse embryo by IHC-Fr.  The tissue was frozen and cut into 10 µm slices on a cyrostat then fixed and blocked prior to incubating with the primary antibody (diluted 1/100) for 90 minutes.  A fluorescent conjugated secondary antibody was incubated with the sample for 30 minutes.  HES-5 is shown in red in the nuclear regions; the blue staining is Hoechst nuclear counter stain.

References for Anti-HES5 antibody (ab25374)

This product has been referenced in:
  • Göttle P  et al. Oligodendroglial maturation is dependent on intracellular protein shuttling. J Neurosci 35:906-19 (2015). ICC/IF ; Rat . Read more (PubMed: 25609610) »
  • Terrinoni A  et al. Role of p63 and the Notch pathway in cochlea development and sensorineural deafness. Proc Natl Acad Sci U S A 110:7300-5 (2013). WB ; Human . Read more (PubMed: 23589895) »

See all 7 Publications for this product

Product Wall

Thank you for your inquiry.

I am happy to confirm that ab25374 is tested and guaranteed for WB with mouse and human samples.

It was tested using standard WB procedure which can be found here:


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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse brain)
Specification mouse brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 26 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Neural cells)
Specification Neural cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 24 2010

The Uniprot localization of Hes5 is listed as Nuclear (by similarity) to other transcriptional repressors. However, Hes5 is widely expressed in the developing nervous system (Akazawa et al., 1992) and in embryos Hes5 is ubiquitously expressed inside...

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