Overview

  • Product name
  • Description
    Rabbit polyclonal to HES7
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide derived from a region between residues 62-112 of human HES7.

    .

  • Positive control
    • Fetal human heart lysate, Human kidney and human pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab26062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation.
  • Involvement in disease
    Defects in HES7 are the cause of spondylocostal dysostosis type 4 (SCDO4) [MIM:613686]. A rare condition of variable severity characterized by vertebral and costal anomalies. The main feature include dwarfism, vertebral fusion, hemivertebrae, posterior rib fusion, reduced rib number, and other rib malformations.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Domain
    Has a particular type of basic domain which includes a helix-interrupting proline.
    The C-terminal WRPW motif is a transcriptional repression motif which is necessary for interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLH factor Hes7 antibody
    • bHLHb37 antibody
    • Class B basic helix loop helix protein 37 antibody
    • Class B basic helix-loop-helix protein 37 antibody
    • Hairy and enhancer of split 7 antibody
    • hes family bHLH transcription factor 7 antibody
    • Hes7 antibody
    • HES7_HUMAN antibody
    • hHes7 antibody
    • SCDO4 antibody
    • Transcription factor HES 7 antibody
    • Transcription factor HES-7 antibody
    see all

Images

  • Anti-HES7 antibody (ab26062) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal human heart lysate

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 25 kDa
    Observed band size : 25,26 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical detection of HES7 using 4 ug/ml ab26062 in paraffin embedded human kidney tissue section. Arrows indicate positively labelled epithelial cells of the renal tubule.
  • Immunohistochemical detection of HES7 using 4 ug/ml ab26062 in paraffin embedded human pancreas tissue section. Arrows indicate positively labelled epithelial cells of the pancreatic acinus.

References

ab26062 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your inquiry.

According to the SwissProt home page (http://www.uniprot.org/uniprot/Q9BYE0) there is only one isoform.

Since I ma not experienced in the field of Transcription factor HES-7 I can only confirm that if bot...

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Thank you for your inquiry.

The exact immunogen sequences for these two antibodies are proprietary information. All the information I can provide is the region as it is stated on the datasheets:

ab26062: The immunogen is within ther...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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