Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody (ab50966)

Overview

  • Product name
    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody
    See all Heterogeneous Nuclear Ribonucleoprotein (A1-like) primary antibodies
  • Description
    Rabbit polyclonal to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    A region within synthetic peptide: MSKSASPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN, corresponding to N terminal amino acids 1-50 of Human Heterogeneous Nuclear Ribonucleoprotein (A1-like)

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human stomach tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Images

  • All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody (ab50966) at 1.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ab50966, at a concentration of 8µg/ml, staining Heterogeneous Nuclear Ribonucleoprotein (A1-like) in paraffin embedded human stomach tissue sections. The positive cells (indicated by arrows) are epithelial cells of fundic glands.

References

ab50966 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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