Overview

  • Product name
  • Description
    Rabbit polyclonal to Hex
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: RLKQENPQSN KKEELESLDS SCDQRQDLPS EQNKGASLDS SQCSPSPASQ , corresponding to amino acids 193-243 of Human Hex

  • Positive control
    • K562 cell lysate, Human muscle tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab42441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
  • Tissue specificity
    Liver and promyelocytic leukemia cell line HL60.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Expressed during hematopoiesis.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hematopoietically expressed homeobox antibody
    • Hematopoietically-expressed homeobox protein HHEX antibody
    • HEX antibody
    • HHEX antibody
    • HHEX_HUMAN antibody
    • HMPH antibody
    • Homeobox hematopoietically expressed antibody
    • Homeobox protein HEX antibody
    • Homeobox protein PRH antibody
    • HOX11L PEN antibody
    • PRH antibody
    • PRHX antibody
    • Proline rich homeodomain containing transcription factor antibody
    see all

Anti-Hex antibody images

  • Ab42441 at 4.0 µg/ml, staining Hex in human skeletal muscle cells by immunohistochemistry, parafin embedded human muscle tissue.
  • Anti-Hex antibody (ab42441) at 0.5 µg/ml + K562 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References for Anti-Hex antibody (ab42441)

This product has been referenced in:
  • Sripichai O  et al. Cytokine-mediated increases in fetal hemoglobin are associated with globin gene histone modification and transcription factor reprogramming. Blood 114:2299-306 (2009). WB ; Human . Read more (PubMed: 19597182) »

See 1 Publication for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification
haematopoietic progenitor cell line HPC-7
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.4% formaldehyde
Detection step
Real-time PCR
Positive control
region known to bind this transcription factor
Negative control
region known to be devoid of transcription factor
Username

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Verified customer

Submitted Jan 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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